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@ARTICLE{Angelova:292156,
author = {A. L. Angelova$^*$ and M. Barf$^*$ and A. Just$^*$ and B.
Leuchs$^*$ and J. Rommelaere$^*$ and G. Ungerechts$^*$},
title = {{H}-1 {P}arvovirus-{I}nduced {O}ncolysis and {T}umor
{M}icroenvironment {I}mmune {M}odulation in a {N}ovel
{H}eterotypic {S}pheroid {M}odel of {C}utaneous {T}-{C}ell
{L}ymphoma.},
journal = {Cancers},
volume = {16},
number = {15},
issn = {2072-6694},
address = {Basel},
publisher = {MDPI},
reportid = {DKFZ-2024-01644},
pages = {2711},
year = {2024},
note = {#EA:D490#LA:D490#},
abstract = {The rat protoparvovirus H-1 (H-1PV) is an oncolytic virus
known for its anticancer properties in laboratory models of
various human tumors, including non-Hodgkin lymphomas (NHL)
of B-cell origin. However, H-1PV therapeutic potential
against hematological malignancies of T-cell origin remains
underexplored. The aim of the present study was to conduct a
pilot preclinical investigation of H-1PV-mediated oncolytic
effects in cutaneous T-cell lymphoma (CTCL), a type of NHL
that is urgently calling for innovative therapies. We
demonstrated H-1PV productive infection and induction of
oncolysis in both classically grown CTCL suspension cultures
and in a novel, in vivo-relevant, heterotypic spheroid
model, but not in healthy donor controls, including
peripheral blood mononuclear cells (PBMCs). H-1PV-mediated
oncolysis of CTCL cells was not prevented by Bcl-2
overexpression and was accompanied by increased
extracellular ATP release. In CTCL spheroid co-cultures with
PBMCs, increased spheroid infiltration with immune cells was
detected upon co-culture treatment with the virus. In
conclusion, our preclinical data show that H-1PV may hold
significant potential as an ingenious viroimmunotherapeutic
drug candidate against CTCL.},
keywords = {cutaneous T-cell lymphoma (CTCL) (Other) / heterotypic CTCL
spheroid (Other) / oncolytic H-1 parvovirus (Other) /
virotherapy (Other)},
cin = {D490 / D430 / Z999},
ddc = {610},
cid = {I:(DE-He78)D490-20160331 / I:(DE-He78)D430-20160331 /
I:(DE-He78)Z999-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39123440},
pmc = {pmc:PMC11311363},
doi = {10.3390/cancers16152711},
url = {https://inrepo02.dkfz.de/record/292156},
}