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@ARTICLE{Will:292356,
      author       = {P. A. Will and F. Taqatqeh and F. Fricke$^*$ and J. E.
                      Berner and N. Lindenblatt and U. Kneser and C. Hirche},
      title        = {{T}issue-engineered cellulose tubes for microvascular and
                      lymphatic reconstruction: {A} translational and feasibility
                      study.},
      journal      = {Journal of plastic, reconstructive $\&$ aesthetic surgery},
      volume       = {97},
      issn         = {1748-6815},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2024-01705},
      pages        = {200-211},
      year         = {2024},
      note         = {2024 Oct;97:200-211},
      abstract     = {Lymphedema microsurgery is an emerging treatment modality,
                      with dissimilar long-term outcomes. One of the main
                      technical challenges in lymphatic microsurgery is the
                      identification and availability of suitable donor vessels
                      for anastomosis. Tissue engineering using biomaterials has
                      demonstrated promise in addressing vessel quality issues in
                      other fields, but its application in microsurgery is still
                      limited.Decellularized cellulose tubes were developed and
                      bioengineered by decellularizing stems of
                      Taraxacum-Ruderalia. The microscopic structure, mechanical
                      properties, and residual DNA content of the cellulose tubes
                      were evaluated. Human and murine skin fibroblasts and dermal
                      lymphatic endothelial cells were isolated and cultured for
                      recellularization studies. Biocompatibility, proliferative
                      capacity, and ex-vivo endothelialization of the cellulose
                      tubes were assessed as potential interposition grafts.
                      Finally, the engineered cellulose tubes were assessed as
                      interposing xenografts for lymphovenous anastomoses (LVA) in
                      an ex-vivo swine limb model.The decellularized cellulose
                      tubes exhibited a suitable microscopic structure, mechanical
                      properties, and low residual DNA content. The tubes showed
                      adequate biocompatibility, supported cell proliferation, and
                      facilitated spontaneous ex-vivo endothelialization of
                      lymphatic endothelial cells. In the swine limb model, LVA
                      using the engineered cellulose tubes was successfully
                      performed.This translational study presents the use of
                      decellularized cellulose tubes as an adjunct for micro and
                      supermicrosurgical reconstruction. The developed tubes
                      demonstrated favorable structural, mechanical, and
                      biocompatible properties, making them a potential candidate
                      for improving long-term outcomes in lymphedema surgical
                      treatment. The next translational step would be trialing the
                      obtained tubes in a microsurgical in-vivo model.},
      keywords     = {Anastomosis, surgical (Other) / Cellulose (Other) /
                      Heterografts (Other) / Lymphedema (Other) / Microsurgery
                      (Other) / Tissue engineering (Other)},
      cin          = {D470},
      ddc          = {610},
      cid          = {I:(DE-He78)D470-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39168030},
      doi          = {10.1016/j.bjps.2024.05.043},
      url          = {https://inrepo02.dkfz.de/record/292356},
}