TY  - JOUR
AU  - Hoa Ho, Kim
AU  - Trapp, Marleen
AU  - Guida, Catello
AU  - Ivanova, Ekaterina
AU  - De Jaime-Soguero, Anchel
AU  - Jabali, Ammar
AU  - Thomas, Christian
AU  - Salasova, Alena
AU  - Bernatík, Ondřej
AU  - Salio, Chiara
AU  - Horschitz, Sandra
AU  - Hasselblatt, Martin
AU  - Sassoe-Pognetto, Marco
AU  - Čajánek, Lukáš
AU  - Ishikawa, Hiroshi
AU  - Schroten, Horst
AU  - Schwerk, Christian
AU  - Acebrón, Sergio P
AU  - Angel, Peter
AU  - Koch, Philipp
AU  - Patrizi, Annarita
TI  - Activation of Wnt/β-catenin signaling is critical for the tumorigenesis of choroid plexus.
JO  - Neuro-Oncology
VL  - 27
IS  - 1
SN  - 1522-8517
CY  - Oxford
PB  - Oxford Univ. Press
M1  - DKFZ-2024-01735
SP  - 106-122
PY  - 2025
N1  - #EA:A320#LA:A320#  / DKFZ/ZMBH Alliance / 2025 Jan 12;27(1):106-122
AB  - Choroid plexus (ChP) is the secretory epithelial structure located in brain ventricles. Choroid plexus tumors (CPTs) are rare neoplasms predominantly occurring in young patients with intensified malignancy in children. CPT treatment is hindered by insufficient knowledge of the tumor pathology and limited availability of valid models.Genomic and transcriptomic data from CPT patients were analyzed to identify the putative pathological pathway. Cellular and molecular techniques were employed to validate bioinformatic results in CPT patient samples. Pharmacologic inhibition of Wnt/β-catenin signaling was assessed in CPT cells. Cell-based assays of ChP cell lines were performed following CRISPR-Cas9-derived knockout and over-expression of Wnt/β-catenin pathway genes. 3D CPT model was generated through CRISPR-Cas9-derived knockout of APC.We discovered that Wnt/β-catenin signaling is activated in human CPTs, likely as a consequence of large-scale chromosomal instability events of the CPT genomes. We demonstrated that CPT-derived cells depend on autocrine Wnt/β-catenin signaling for survival. Constitutive Wnt/β-catenin pathway activation, either through knock-out of the negative regulator APC or overexpression of the ligand WNT3A, induced tumorigenic properties in ChP 2D in vitro models. Increased activation of Wnt/β-catenin pathway in ChP organoids, through treatment with a potent GSK3β inhibitor, reduced the differentiation of mature ChP epithelia cells. Remarkably, the depletion of APC was sufficient to induce the oncogenic transformation of ChP organoids.Our research identifies Wnt/β-catenin signaling as a critical driver of CPT tumorigenesis and provides the first 3D in vitro model for future pathological and therapeutic studies of CPT.
KW  - APC (Other)
KW  - Brain tumor (Other)
KW  - Wnt signaling (Other)
KW  - choroid plexus organoid (Other)
KW  - rare childhood cancer (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39215664
DO  - DOI:10.1093/neuonc/noae176
UR  - https://inrepo02.dkfz.de/record/292488
ER  -