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000294334 0247_ $$2doi$$a10.1016/j.crmeth.2024.100880
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000294334 1001_ $$aLazarewicz, Natalia$$b0
000294334 245__ $$aAccurate and sensitive interactome profiling using a quantitative protein-fragment complementation assay.
000294334 260__ $$aCambridge, MA$$bCell Press$$c2024
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000294334 520__ $$aAn accurate description of protein-protein interaction (PPI) networks is key to understanding the molecular mechanisms underlying cellular systems. Here, we constructed genome-wide libraries of yeast strains to systematically probe protein-protein interactions using NanoLuc Binary Technology (NanoBiT), a quantitative protein-fragment complementation assay (PCA) based on the NanoLuc luciferase. By investigating an array of well-documented PPIs as well as the interactome of four proteins with varying levels of characterization-including the well-studied nonsense-mediated mRNA decay (NMD) regulator Upf1 and the SCF complex subunits Cdc53 and Met30-we demonstrate that ratiometric NanoBiT measurements enable highly precise and sensitive mapping of PPIs. This work provides a foundation for employing NanoBiT in the assembly of more comprehensive and accurate protein interaction maps as well as in their functional investigation.
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000294334 650_7 $$2Other$$aCP: Systems biology
000294334 650_7 $$2Other$$aCdc53
000294334 650_7 $$2Other$$aIrc20
000294334 650_7 $$2Other$$aMet30
000294334 650_7 $$2Other$$aNam7
000294334 650_7 $$2Other$$aNanoBiT
000294334 650_7 $$2Other$$aSaccharomyces cerevisiae
000294334 650_7 $$2Other$$aUpf1
000294334 650_7 $$2Other$$abudding yeast
000294334 650_7 $$2Other$$ainteractome
000294334 650_7 $$2Other$$aprotein-protein interaction
000294334 650_7 $$2NLM Chemicals$$aSaccharomyces cerevisiae Proteins
000294334 650_7 $$0EC 1.13.12.-$$2NLM Chemicals$$aLuciferases
000294334 650_2 $$2MeSH$$aProtein Interaction Mapping: methods
000294334 650_2 $$2MeSH$$aSaccharomyces cerevisiae: genetics
000294334 650_2 $$2MeSH$$aSaccharomyces cerevisiae: metabolism
000294334 650_2 $$2MeSH$$aProtein Interaction Maps
000294334 650_2 $$2MeSH$$aSaccharomyces cerevisiae Proteins: metabolism
000294334 650_2 $$2MeSH$$aSaccharomyces cerevisiae Proteins: genetics
000294334 650_2 $$2MeSH$$aLuciferases: genetics
000294334 650_2 $$2MeSH$$aLuciferases: metabolism
000294334 7001_ $$aLe Dez, Gaëlle$$b1
000294334 7001_ $$aCerjani, Romina$$b2
000294334 7001_ $$aRuneshaw, Lunelys$$b3
000294334 7001_ $$aMeurer, Matthias$$b4
000294334 7001_ $$0P:(DE-He78)03ae15a30a7fa7191475148bf4e7f581$$aKnop, Michael$$b5$$udkfz
000294334 7001_ $$aWysocki, Robert$$b6
000294334 7001_ $$aRabut, Gwenaël$$b7
000294334 773__ $$0PERI:(DE-600)3091714-1$$a10.1016/j.crmeth.2024.100880$$gVol. 4, no. 10, p. 100880 -$$n10$$p100880$$tCell reports / Methods$$v4$$x2667-2375$$y2024
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