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@ARTICLE{Lazarewicz:294334,
      author       = {N. Lazarewicz and G. Le Dez and R. Cerjani and L. Runeshaw
                      and M. Meurer and M. Knop$^*$ and R. Wysocki and G. Rabut},
      title        = {{A}ccurate and sensitive interactome profiling using a
                      quantitative protein-fragment complementation assay.},
      journal      = {Cell reports / Methods},
      volume       = {4},
      number       = {10},
      issn         = {2667-2375},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2024-02167},
      pages        = {100880},
      year         = {2024},
      note         = {DKFZ-ZMBH Alliance},
      abstract     = {An accurate description of protein-protein interaction
                      (PPI) networks is key to understanding the molecular
                      mechanisms underlying cellular systems. Here, we constructed
                      genome-wide libraries of yeast strains to systematically
                      probe protein-protein interactions using NanoLuc Binary
                      Technology (NanoBiT), a quantitative protein-fragment
                      complementation assay (PCA) based on the NanoLuc luciferase.
                      By investigating an array of well-documented PPIs as well as
                      the interactome of four proteins with varying levels of
                      characterization-including the well-studied
                      nonsense-mediated mRNA decay (NMD) regulator Upf1 and the
                      SCF complex subunits Cdc53 and Met30-we demonstrate that
                      ratiometric NanoBiT measurements enable highly precise and
                      sensitive mapping of PPIs. This work provides a foundation
                      for employing NanoBiT in the assembly of more comprehensive
                      and accurate protein interaction maps as well as in their
                      functional investigation.},
      keywords     = {Protein Interaction Mapping: methods / Saccharomyces
                      cerevisiae: genetics / Saccharomyces cerevisiae: metabolism
                      / Protein Interaction Maps / Saccharomyces cerevisiae
                      Proteins: metabolism / Saccharomyces cerevisiae Proteins:
                      genetics / Luciferases: genetics / Luciferases: metabolism /
                      CP: Systems biology (Other) / Cdc53 (Other) / Irc20 (Other)
                      / Met30 (Other) / Nam7 (Other) / NanoBiT (Other) /
                      Saccharomyces cerevisiae (Other) / Upf1 (Other) / budding
                      yeast (Other) / interactome (Other) / protein-protein
                      interaction (Other) / Saccharomyces cerevisiae Proteins (NLM
                      Chemicals) / Luciferases (NLM Chemicals)},
      cin          = {A260},
      ddc          = {610},
      cid          = {I:(DE-He78)A260-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39437715},
      doi          = {10.1016/j.crmeth.2024.100880},
      url          = {https://inrepo02.dkfz.de/record/294334},
}