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001     294334
005     20241028182247.0
024 7 _ |a 10.1016/j.crmeth.2024.100880
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100 1 _ |a Lazarewicz, Natalia
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245 _ _ |a Accurate and sensitive interactome profiling using a quantitative protein-fragment complementation assay.
260 _ _ |a Cambridge, MA
|c 2024
|b Cell Press
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520 _ _ |a An accurate description of protein-protein interaction (PPI) networks is key to understanding the molecular mechanisms underlying cellular systems. Here, we constructed genome-wide libraries of yeast strains to systematically probe protein-protein interactions using NanoLuc Binary Technology (NanoBiT), a quantitative protein-fragment complementation assay (PCA) based on the NanoLuc luciferase. By investigating an array of well-documented PPIs as well as the interactome of four proteins with varying levels of characterization-including the well-studied nonsense-mediated mRNA decay (NMD) regulator Upf1 and the SCF complex subunits Cdc53 and Met30-we demonstrate that ratiometric NanoBiT measurements enable highly precise and sensitive mapping of PPIs. This work provides a foundation for employing NanoBiT in the assembly of more comprehensive and accurate protein interaction maps as well as in their functional investigation.
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650 _ 7 |a CP: Systems biology
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650 _ 7 |a Cdc53
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650 _ 7 |a Irc20
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650 _ 7 |a Met30
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650 _ 7 |a Nam7
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650 _ 7 |a NanoBiT
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650 _ 7 |a Saccharomyces cerevisiae
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650 _ 7 |a Upf1
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650 _ 7 |a budding yeast
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650 _ 7 |a interactome
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650 _ 7 |a protein-protein interaction
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650 _ 7 |a Saccharomyces cerevisiae Proteins
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650 _ 7 |a Luciferases
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650 _ 2 |a Protein Interaction Mapping: methods
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae: genetics
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae: metabolism
|2 MeSH
650 _ 2 |a Protein Interaction Maps
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae Proteins: metabolism
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae Proteins: genetics
|2 MeSH
650 _ 2 |a Luciferases: genetics
|2 MeSH
650 _ 2 |a Luciferases: metabolism
|2 MeSH
700 1 _ |a Le Dez, Gaëlle
|b 1
700 1 _ |a Cerjani, Romina
|b 2
700 1 _ |a Runeshaw, Lunelys
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700 1 _ |a Meurer, Matthias
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700 1 _ |a Knop, Michael
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700 1 _ |a Wysocki, Robert
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700 1 _ |a Rabut, Gwenaël
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773 _ _ |a 10.1016/j.crmeth.2024.100880
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