%0 Journal Article
%A Soleder, Stefan
%A Gengenbacher, Nicolas
%A Mogler, Carolin
%A Eckstein, Markus
%A Runge, Anja
%A Kriegmair, Maximilian C
%A Augustin, Hellmut
%T Development of a novel immunocompetent murine tumor model for urothelial carcinoma using in vivo electroporation.
%J Scientific reports
%V 14
%N 1
%@ 2045-2322
%C [London]
%I Macmillan Publishers Limited, part of Springer Nature
%M DKFZ-2024-02168
%P 25619
%D 2024
%Z #EA:A190#LA:A190#
%X A lack of advanced preclinical mouse tumor models impedes the progress in urothelial carcinoma research. We present here a novel fast, robust, reliable, and highly reproducible model for the genetic induction of bladder cancer in immunocompetent mice. Different sets of oncogenic transposons (Cmyc, Kras) and Cre drivers were transfected into the murine bladder wall of two different genetic backgrounds (Trp53fl/fl and BrafV600E, Ptenfl/fl, Ctnnb1exon3-fl/fl). Transfection was carried out using in vivo electroporation of the bladder after surgical exploration and transmural or transurethral intravesical plasmid injection. Up to 100
%K Animals
%K Electroporation: methods
%K Mice
%K Urinary Bladder Neoplasms: pathology
%K Urinary Bladder Neoplasms: genetics
%K Disease Models, Animal
%K Female
%K Carcinoma, Transitional Cell: pathology
%K Carcinoma, Transitional Cell: genetics
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39463382
%R 10.1038/s41598-024-77178-z
%U https://inrepo02.dkfz.de/record/294335