TY  - JOUR
AU  - Soleder, Stefan
AU  - Gengenbacher, Nicolas
AU  - Mogler, Carolin
AU  - Eckstein, Markus
AU  - Runge, Anja
AU  - Kriegmair, Maximilian C
AU  - Augustin, Hellmut
TI  - Development of a novel immunocompetent murine tumor model for urothelial carcinoma using in vivo electroporation.
JO  - Scientific reports
VL  - 14
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2024-02168
SP  - 25619
PY  - 2024
N1  - #EA:A190#LA:A190#
AB  - A lack of advanced preclinical mouse tumor models impedes the progress in urothelial carcinoma research. We present here a novel fast, robust, reliable, and highly reproducible model for the genetic induction of bladder cancer in immunocompetent mice. Different sets of oncogenic transposons (Cmyc, Kras) and Cre drivers were transfected into the murine bladder wall of two different genetic backgrounds (Trp53fl/fl and BrafV600E, Ptenfl/fl, Ctnnb1exon3-fl/fl). Transfection was carried out using in vivo electroporation of the bladder after surgical exploration and transmural or transurethral intravesical plasmid injection. Up to 100
KW  - Animals
KW  - Electroporation: methods
KW  - Mice
KW  - Urinary Bladder Neoplasms: pathology
KW  - Urinary Bladder Neoplasms: genetics
KW  - Disease Models, Animal
KW  - Female
KW  - Carcinoma, Transitional Cell: pathology
KW  - Carcinoma, Transitional Cell: genetics
LB  - PUB:(DE-HGF)16
C6  - pmid:39463382
DO  - DOI:10.1038/s41598-024-77178-z
UR  - https://inrepo02.dkfz.de/record/294335
ER  -