% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Soleder:294335,
      author       = {S. Soleder$^*$ and N. Gengenbacher$^*$ and C. Mogler and M.
                      Eckstein and A. Runge$^*$ and M. C. Kriegmair and H.
                      Augustin$^*$},
      title        = {{D}evelopment of a novel immunocompetent murine tumor model
                      for urothelial carcinoma using in vivo electroporation.},
      journal      = {Scientific reports},
      volume       = {14},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2024-02168},
      pages        = {25619},
      year         = {2024},
      note         = {#EA:A190#LA:A190#},
      abstract     = {A lack of advanced preclinical mouse tumor models impedes
                      the progress in urothelial carcinoma research. We present
                      here a novel fast, robust, reliable, and highly reproducible
                      model for the genetic induction of bladder cancer in
                      immunocompetent mice. Different sets of oncogenic
                      transposons (Cmyc, Kras) and Cre drivers were transfected
                      into the murine bladder wall of two different genetic
                      backgrounds (Trp53fl/fl and BrafV600E, Ptenfl/fl,
                      Ctnnb1exon3-fl/fl). Transfection was carried out using in
                      vivo electroporation of the bladder after surgical
                      exploration and transmural or transurethral intravesical
                      plasmid injection. Up to $100\%$ of animals developed
                      urothelial carcinomas of the bladder. Time to tumor onset
                      ranged from 16 to 97 days with a median of approximately 23
                      days in the fastest groups. Histological examination
                      identified orthotopic urothelial carcinomas in most cases,
                      in some experimental groups up to $100\%.$ The resulting
                      tumors were highly invasive and often metastatic. Metastases
                      were found in up to $100\%$ of tumor bearing mice per group.
                      Taken together, this study establishes the
                      proof-of-principle that in vivo electroporation can be
                      versatilely employed as a reliable, fast, and robust method
                      for the highly reproducible induction of urothelial
                      carcinomas in the murine bladder wall. This novel murine
                      tumor model could pave the way towards more easily modelling
                      subtype specific urothelial carcinomas in mice.},
      keywords     = {Animals / Electroporation: methods / Mice / Urinary Bladder
                      Neoplasms: pathology / Urinary Bladder Neoplasms: genetics /
                      Disease Models, Animal / Female / Carcinoma, Transitional
                      Cell: pathology / Carcinoma, Transitional Cell: genetics},
      cin          = {A190},
      ddc          = {600},
      cid          = {I:(DE-He78)A190-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39463382},
      doi          = {10.1038/s41598-024-77178-z},
      url          = {https://inrepo02.dkfz.de/record/294335},
}