% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Willemsen:294378,
      author       = {S. Willemsen and F. A. Y. Yengej and J. Puschhof$^*$ and M.
                      B. Rookmaaker and M. C. Verhaar and J. van Es and J. Beumer
                      and H. Clevers},
      title        = {{A} comprehensive transcriptome characterization of
                      individual nuclear receptor pathways in the human small
                      intestine.},
      journal      = {Proceedings of the National Academy of Sciences of the
                      United States of America},
      volume       = {121},
      number       = {45},
      issn         = {0027-8424},
      address      = {Washington, DC},
      publisher    = {National Acad. of Sciences},
      reportid     = {DKFZ-2024-02211},
      pages        = {e2411189121},
      year         = {2024},
      abstract     = {Nuclear receptors (NRs) are widely expressed transcription
                      factors that bind small, lipophilic compounds and regulate
                      diverse biological processes. In the small intestine, NRs
                      are known to act as sensors that control transcriptional
                      responses to endogenous and exogenous signals, yet their
                      downstream effects have not been characterized extensively.
                      Here, we investigate the activation of six different NRs
                      individually in human intestinal organoids using small
                      molecules agonists. We observe changes in key enterocyte
                      functions such as lipid, glucose, and amino acid absorption,
                      the regulation of electrolyte balance, and drug metabolism.
                      Our findings reinforce PXR, LXR, FXR, and PPARα as
                      regulators of lipid absorption. Furthermore, known hepatic
                      effects of AHR and VDR activation were recapitulated in the
                      human small intestine. Finally, we identify unique target
                      genes for intestinal PXR activation (ERG28, TMEM97, and
                      TM7SF2), LXR activation (RAB6B), and VDR activation (CA12).
                      This study provides an unbiased and comprehensive
                      transcriptomic description of individual NR pathways in the
                      human small intestine. By gaining a deeper understanding of
                      the effects of individual NRs, we might better harness their
                      pharmacological and therapeutic potential.},
      keywords     = {Humans / Intestine, Small: metabolism / Receptors,
                      Cytoplasmic and Nuclear: metabolism / Receptors, Cytoplasmic
                      and Nuclear: genetics / Transcriptome / Receptors,
                      Calcitriol: metabolism / Receptors, Calcitriol: genetics /
                      Liver X Receptors: metabolism / Liver X Receptors: genetics
                      / Liver X Receptors: agonists / Organoids: metabolism /
                      Signal Transduction / Lipid Metabolism: genetics /
                      Enterocytes: metabolism / Pregnane X Receptor: metabolism /
                      Pregnane X Receptor: genetics / Gene Expression Regulation:
                      drug effects / lipid absorption (Other) / nuclear receptors
                      (Other) / organoids (Other) / small intestine (Other) /
                      Receptors, Cytoplasmic and Nuclear (NLM Chemicals) /
                      Receptors, Calcitriol (NLM Chemicals) / Liver X Receptors
                      (NLM Chemicals) / VDR protein, human (NLM Chemicals) /
                      Pregnane X Receptor (NLM Chemicals)},
      cin          = {D300},
      ddc          = {500},
      cid          = {I:(DE-He78)D300-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39475639},
      doi          = {10.1073/pnas.2411189121},
      url          = {https://inrepo02.dkfz.de/record/294378},
}