TY  - JOUR
AU  - Böhl, Elias
AU  - Raddatz, Günter
AU  - Roy, Suki
AU  - Huang, Lingzhi
AU  - Sandhu, Jasrene Kaur
AU  - Igwe, Emeka Ignatius
AU  - Rodríguez-Paredes, Manuel
AU  - Böhl, Florian
AU  - Lyko, Frank
TI  - Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing.
JO  - Journal of biotechnology
VL  - 396
SN  - 0168-1656
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - DKFZ-2024-02226
SP  - 72-79
PY  - 2024
N1  - #EA:A130#LA:A130# / 2024 Oct 31:396:72-79 / DKFZ-ZMBH Alliance
AB  - CHO cells are major hosts for the industrial production of therapeutic proteins and their production stability is of considerable economic significance. It is widely known that CHO cells can rapidly acquire genetic alterations, which affects their genetic homogeneity over time. However, the role of non-genetic mechanisms, including epigenetic mechanisms such as DNA methylation, remains poorly understood. We have now used whole-genome bisulfite sequencing to establish single-base methylation maps of eight independent CHO cell lines. Our results identify CpG islands and low-methylated regions as conserved elements with dynamic DNA methylation. Interestingly, methylation patterns were found to cluster clearly along the three main branches of CHO evolution, with no directional changes over short culture periods. Furthermore, multi-ome single-cell sequencing of 9,833 nuclei from three independent cultures revealed dynamic subpopulation structures characterized by robust expression differences in pathways related to protein production. Our findings thus provide novel insights into the epigenetic landscape and heterogeneity of CHO cells and support the development of epigenetic biomarkers that trace the emergence of subpopulations in CHO cultures.
KW  - CHO cells (Other)
KW  - DNA methylation (Other)
KW  - Epigenetics (Other)
KW  - Evolution (Other)
KW  - single-cell sequencing (Other)
KW  - subpopulations (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39488254
DO  - DOI:10.1016/j.jbiotec.2024.10.012
UR  - https://inrepo02.dkfz.de/record/294397
ER  -