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@PHDTHESIS{Daugelaite:294445,
      author       = {K. Daugelaite$^*$},
      title        = {{R}egulation of hormonal stimulation and ageing in female
                      reproductive tract},
      school       = {Universität Heidelberg},
      type         = {Dissertation},
      reportid     = {DKFZ-2024-02270},
      pages        = {104 p.},
      year         = {2024},
      note         = {Dissertation, Universität Heidelberg, 2024},
      abstract     = {In this thesis, I present four scientific projects that I
                      lead or co-lead during my thesis, each interrogating
                      different aspects of regulation within the female
                      reproductive tract. The first project addresses critical
                      gaps in our understanding of oocyte and granulosa cell
                      interactions within cumulus-oocyte complexes (COCs) in the
                      context of superovulation and ageing. I adapted
                      developmental biology techniques to enable precise
                      comparisons between individually tracked oocyte-granulosa
                      cell pairs and naturally aged COCs. Additionally, I designed
                      a prospective mouse IVF model to non-invasively predict
                      embryo development trajectory using granulosa cell
                      transcriptomes, addressing limitations of retrospective
                      studies. The second project explores the evolutionary
                      transcriptional divergence of oocytes across different mouse
                      species, contributing to our understanding of oocyte
                      evolution - a field predominantly focused on male gametes.
                      Preliminary results suggest nuanced patterns of
                      transcriptional divergence among species. In the third
                      project, I contributed experimentally to a larger scientific
                      project that investigates of the role of fibroblasts in
                      shaping inflammation and extracellular matrix remodelling in
                      the female reproductive tract during ageing. The results
                      emphasize the contribution of fibroblasts to age-related
                      fibrosis and chronic tissue inflammation. Finally, the last
                      project involved collecting extensive datasets of mouse
                      single cell RNA sequencing and spatial transcriptomics data
                      to provide insights into female reproductive tract ageing
                      after repeated hormonal stimulations, complemented by
                      additional experiments in mouse and human. This research
                      project will have significant implications for understanding
                      the long-term effects of hormonal stimulation on
                      reproductive tissue health and the broader mechanisms of
                      reproductive ageing.},
      cin          = {B270},
      cid          = {I:(DE-He78)B270-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)11},
      url          = {https://inrepo02.dkfz.de/record/294445},
}