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@ARTICLE{Sala:294463,
author = {C. Sala and M. Würtz and E. S. Atorino and A. Neuner and
P. J. Partscht$^*$ and T. Hoffmann and S. Eustermann and E.
Schiebel},
title = {{A}n interaction network of inner centriole proteins
organised by {POC}1{A}-{POC}1{B} heterodimer crosslinks
ensures centriolar integrity.},
journal = {Nature Communications},
volume = {15},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DKFZ-2024-02288},
pages = {9857},
year = {2024},
note = {(DKFZ)-ZMBH Allianz},
abstract = {Centriole integrity, vital for cilia formation and
chromosome segregation, is crucial for human health. The
inner scaffold within the centriole lumen composed of the
proteins POC1B, POC5 and FAM161A is key to this integrity.
Here, we provide an understanding of the function of inner
scaffold proteins. We demonstrate the importance of an
interaction network organised by POC1A-POC1B heterodimers
within the centriole lumen, where the WD40 domain of POC1B
localises close to the centriole wall, while the
POC5-interacting WD40 of POC1A resides in the centriole
lumen. The POC1A-POC5 interaction and POC5 tetramerization
are essential for inner scaffold formation and centriole
stability. The microtubule binding proteins FAM161A and MDM1
by binding to POC1A-POC1B, likely positioning the POC5
tetramer near the centriole wall. Disruption of POC1A or
POC1B leads to centriole microtubule defects and deletion of
both genes causes centriole disintegration. These findings
provide insights into organisation and function of the inner
scaffold.},
cin = {D250},
ddc = {500},
cid = {I:(DE-He78)D250-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39543170},
doi = {10.1038/s41467-024-54247-5},
url = {https://inrepo02.dkfz.de/record/294463},
}