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@ARTICLE{Eid:294676,
      author       = {M. Eid$^*$ and U. Barayeu$^*$ and K. Sulková$^*$ and C.
                      Aranda-Vallejo$^*$ and T. Dick$^*$},
      title        = {{U}sing the heme peroxidase {APEX}2 to probe intracellular
                      {H}2{O}2 flux and diffusion.},
      journal      = {Nature Communications},
      volume       = {15},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {DKFZ-2024-02411},
      pages        = {1239},
      year         = {2024},
      note         = {#EA:A160#LA:A160# / DKFZ-ZMBH Alliance},
      abstract     = {Currently available genetically encoded H2O2 probes report
                      on the thiol redox state of the probe, which means that they
                      reflect the balance between probe thiol oxidation and
                      reduction. Here we introduce the use of the engineered heme
                      peroxidase APEX2 as a thiol-independent chemogenetic H2O2
                      probe that directly and irreversibly converts H2O2 molecules
                      into either fluorescent or luminescent signals. We
                      demonstrate sensitivity, specificity, and the ability to
                      quantitate endogenous H2O2 turnover. We show how the probe
                      can be used to detect changes in endogenous H2O2 generation
                      and to assess the roles and relative contributions of
                      endogenous H2O2 scavengers. Furthermore, APEX2 can be used
                      to study H2O2 diffusion inside the cytosol. Finally, APEX2
                      reveals the impact of commonly used alkylating agents and
                      cell lysis protocols on cellular H2O2 generation.},
      keywords     = {Heme / Hydrogen Peroxide / Oxidation-Reduction /
                      Peroxidases: chemistry / Peroxidases: metabolism /
                      Sulfhydryl Compounds / Heme (NLM Chemicals) / Hydrogen
                      Peroxide (NLM Chemicals) / Peroxidases (NLM Chemicals) /
                      Sulfhydryl Compounds (NLM Chemicals)},
      cin          = {A160},
      ddc          = {500},
      cid          = {I:(DE-He78)A160-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311) / Redox
                      Relays - Detecting, understanding and exploiting
                      intracellular redox signaling relays (742039)},
      pid          = {G:(DE-HGF)POF4-311 / G:(EU-Grant)742039},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38336829},
      pmc          = {pmc:PMC10858230},
      doi          = {10.1038/s41467-024-45511-9},
      url          = {https://inrepo02.dkfz.de/record/294676},
}