TY  - JOUR
AU  - Jessa, Selin
AU  - De Cola, Antonella
AU  - Chandarana, Bhavyaa
AU  - McNicholas, Michael
AU  - Hébert, Steven
AU  - Ptack, Adam
AU  - Faury, Damien
AU  - Tsai, Jessica W
AU  - Korshunov, Andrey
AU  - Phoenix, Timothy N
AU  - Ellezam, Benjamin
AU  - Jones, David
AU  - Taylor, Michael D
AU  - Bandopadhayay, Pratiti
AU  - Pathania, Manav
AU  - Jabado, Nada
AU  - Kleinman, Claudia L
TI  - FOXR2 targets LHX6+/DLX+ neural lineages to drive CNS neuroblastoma.
JO  - Cancer research
VL  - 85
IS  - 2
SN  - 0099-7013
CY  - Philadelphia, Pa.
PB  - AACR
M1  - DKFZ-2024-02456
SP  - 231-250
PY  - 2025
N1  - 2025 Jan 15;85(2):231-250
AB  - Central nervous system neuroblastoma with FOXR2 activation (NB-FOXR2) is a high-grade tumor of the brain hemispheres and a newly identified molecular entity. Tumors express dual neuronal and glial markers, leading to frequent misdiagnoses, and limited information exists on the role of FOXR2 in their genesis. To identify their cellular origins, we profiled the transcriptomes of NB-FOXR2 tumors at the bulk and single-cell levels and integrated these profiles with large single-cell references of the normal brain. NB-FOXR2 tumors mapped to LHX6+/DLX+ lineages derived from the medial ganglionic eminence, a progenitor domain in the ventral telencephalon. In vivo prenatal Foxr2 targeting to the ganglionic eminences in mice induced postnatal cortical tumors recapitulating human NB-FOXR2 specific molecular signatures. Profiling of FOXR2 binding on chromatin in murine models revealed an association with ETS transcriptional networks, as well as direct binding of FOXR2 at key transcription factors that coordinate initiation of gliogenesis. These data indicate that NB-FOXR2 originate from LHX6+/DLX+ interneuron lineages, a lineage-of-origin distinct from that of other FOXR2-driven brain tumors, highlight the susceptibility of ventral telencephalon-derived interneurons to FOXR2-driven oncogenesis, and suggest that FOXR2-induced activation of glial programs may explain the mixed neuronal and oligodendroglial features in these tumors. More broadly, this work underscores systematic profiling of brain development as an efficient approach to orient oncogenic targeting for in vivo modeling, critical for the study of rare tumors and development of therapeutics.
LB  - PUB:(DE-HGF)16
C6  - pmid:39495206
DO  - DOI:10.1158/0008-5472.CAN-24-2248
UR  - https://inrepo02.dkfz.de/record/294723
ER  -