TY - JOUR AU - Kwiatkowski, Janet L AU - Walters, Mark C AU - Hongeng, Suradej AU - Yannaki, Evangelia AU - Kulozik, Andreas AU - Kunz, Joachim B AU - Sauer, Martin G AU - Thrasher, Adrian J AU - Thuret, Isabelle AU - Lal, Ashutosh AU - Tao, Ge AU - Ali, Shamshad AU - Thakar, Himal L AU - Elliot, Heidi AU - Lodaya, Ankit AU - Lee, Ji AU - Colvin, Richard A AU - Locatelli, Franco AU - Thompson, Alexis A TI - Betibeglogene autotemcel gene therapy in patients with transfusion-dependent, severe genotype β-thalassaemia (HGB-212): a non-randomised, multicentre, single-arm, open-label, single-dose, phase 3 trial. JO - The lancet VL - 404 IS - 10468 SN - 0140-6736 CY - London [u.a.] PB - Elsevier M1 - DKFZ-2024-02457 SP - 2175 - 2186 PY - 2024 AB - Transfusion-dependent β-thalassaemia (TDT) is a severe disease, resulting in lifelong blood transfusions, iron overload, and associated complications. Betibeglogene autotemcel (beti-cel) gene therapy uses autologous haematopoietic stem and progenitor cells (HSPCs) transduced with BB305 lentiviral vector to enable transfusion independence.HGB-212 was a non-randomised, multicentre, single-arm, open-label, phase 3 study of beti-cel in patients with TDT conducted at eight centres in France, Germany, Greece, Italy, the UK, and the USA. Patients with β0/β0, β0/β+IVS-I-110, or β+IVS-I-110/β+IVS-I-110 genotypes, clinically stable TDT, and a transfusion history of at least 100 mL/kg per year of packed red blood cells (pRBCs) or at least eight transfusions of pRBCs per year in the 2 years before enrolment were eligible for participation. After undergoing HSPC mobilisation and busulfan-based, pharmacokinetic-adjusted myeloablative conditioning, patients were infused with beti-cel and followed up for 24 months. The primary efficacy outcome was transfusion independence, defined as weighted average haemoglobin level of 9 g/dL or above without pRBC transfusions for 12 or more months. The primary outcome was measured in all patients who received an infusion of beti-cel (transplant population); safety was evaluated in all patients who initiated study treatment (intention-to-treat population). Patients were eligible to enrol in the ongoing 13-year long-term follow-up study (for a total of 15 years), LTF-303 (registered at ClinicalTrials.gov, NCT02633943). This trial, HGB-212, was registered at ClinicalTrials.gov (NCT03207009), and is complete.From June 8, 2017, to March 12, 2020, 20 patients were screened for eligibility. One patient was ineligible and one withdrew consent before HSPC mobilisation and myeloablative conditioning. Of the 18 patients who received beti-cel, ten (56 LB - PUB:(DE-HGF)16 C6 - pmid:39527960 DO - DOI:10.1016/S0140-6736(24)01884-1 UR - https://inrepo02.dkfz.de/record/294724 ER -