% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Mathioudaki:294790,
      author       = {A. Mathioudaki$^*$ and X. Wang and D. Sedloev and R. Huth
                      and A. Kamal and M. Hundemer and Y. Liu and S. Vasileiou and
                      P. Lulla and C. Müller-Tidow and P. Dreger and T. Luft and
                      T. Sauer and M. Schmitt and J. B. Zaugg and C. Pabst},
      title        = {{T}he remission status of {AML} patients after allo-{HCT}
                      is associated with a distinct single-cell bone marrow
                      {T}-cell signature},
      journal      = {Blood},
      volume       = {143},
      number       = {13},
      issn         = {0006-4971},
      address      = {Washington, DC},
      publisher    = {American Society of Hematology},
      reportid     = {DKFZ-2024-02502},
      pages        = {1269 - 1281},
      year         = {2024},
      abstract     = {Acute myeloid leukemia (AML) is a hematologic malignancy
                      for which allogeneic hematopoietic cell transplantation
                      (allo-HCT) often remains the only curative therapeutic
                      approach. However, incapability of T cells to recognize and
                      eliminate residual leukemia stem cells might lead to an
                      insufficient graft-versus-leukemia (GVL) effect and relapse.
                      Here, we performed single-cell RNA-sequencing (scRNA-seq) on
                      bone marrow (BM) T lymphocytes and CD34+ cells of 6 patients
                      with AML 100 days after allo-HCT to identify T-cell
                      signatures associated with either imminent relapse (REL) or
                      durable complete remission (CR). We observed a higher
                      frequency of cytotoxic CD8+ effector and gamma delta (γδ)
                      T cells in CR vs REL samples. Pseudotime and gene regulatory
                      network analyses revealed that CR CD8+ T cells were more
                      advanced in maturation and had a stronger cytotoxicity
                      signature, whereas REL samples were characterized by
                      inflammatory tumor necrosis factor/NF-κB signaling and an
                      immunosuppressive milieu. We identified ADGRG1/GPR56 as a
                      surface marker enriched in CR CD8+ T cells and confirmed in
                      a CD33-directed chimeric antigen receptor T cell/AML
                      coculture model that GPR56 becomes upregulated on T cells
                      upon antigen encounter and elimination of AML cells. We show
                      that GPR56 continuously increases at the protein level on
                      CD8+ T cells after allo-HCT and confirm faster interferon
                      gamma (IFN-γ) secretion upon re-exposure to matched, but
                      not unmatched, recipient AML cells in the GPR56+ vs GPR56-
                      CD8+ T-cell fraction. Together, our data provide a
                      single-cell reference map of BM-derived T cells after
                      allo-HCT and propose GPR56 expression dynamics as a
                      surrogate for antigen encounter after allo-HCT.},
      cin          = {B450},
      ddc          = {610},
      cid          = {I:(DE-He78)B450-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1182/blood.2023021815},
      url          = {https://inrepo02.dkfz.de/record/294790},
}