%0 Journal Article
%A Rashidipour, Marzieh
%A Abbaszadeh, Saber
%A Birjandi, Mehdi
%A Pajouhi, Naser
%A Ahmadi Somaghian, Shahram
%A Goudarzi, Gholamreza
%A Shahryarhesami, Soroosh
%A Moradi Sarabi, Mostafa
%A Babaeenezhad, Esmaeel
%T Antimicrobial activity and cytotoxic and epigenetic effects of tannic acid-loaded chitosan nanoparticles.
%J Scientific reports
%V 14
%N 1
%@ 2045-2322
%C [London]
%I Macmillan Publishers Limited, part of Springer Nature
%M DKFZ-2024-02539
%P 30405
%D 2024
%X Tannic acid (TA) is a potent antitumor agent, but its low bioavailability and absorption limit its use. In this study, it was loaded into chitosan-based nanoparticles (Chi-NPs) to overcome these limitations and to improve its antimicrobial and anticancer activities. TA-loaded Chi-NPs (Chi-TA-NPs) were synthesized using the ionic gelation method and physicochemically characterized by FE-SEM, FTIR, XRD, PDI, DLS, and zeta potential analysis. Additionally, the antimicrobial activity of Chi-TA-NPs against two G+ bacterial strains, two G- bacterial strains, and a fungal strain (Candida albicans) was investigated using the microbroth dilution method. MTT assay was used to examine the cytotoxic effects of Chi-TA-NPs on HepG2 cells. The expression of DNA methyltransferase 1 (DNMT1), DNMT3A, and DNMT3B was examined in HepG2 cells using RT-qPCR. The amount of 5-methylcytosine in the HepG2 cell-derived genomic DNA was measured using ELISA. FE-SEM micrographs showed the loading of TA into the chitosan-based formulation. The peaks detected in the XRD and FTIR analyses confirmed the formation of the Chi-TA-NPs. The PDI value (0.247 ± 0.03), size (567.0 ± 25.84 nm), and zeta potential (17.0 ± 5.86 mV) confirmed the relative stability of Chi-TA-NPs. A constant release profile in line with the Korsmeyer-Peppas model was detected for Chi-TA-NPs, such that approximately 44
%K Chitosan: chemistry
%K Chitosan: pharmacology
%K Humans
%K Tannins: chemistry
%K Tannins: pharmacology
%K Nanoparticles: chemistry
%K Hep G2 Cells
%K Anti-Infective Agents: pharmacology
%K Anti-Infective Agents: chemistry
%K Epigenesis, Genetic: drug effects
%K Candida albicans: drug effects
%K Microbial Sensitivity Tests
%K Antineoplastic Agents: pharmacology
%K Antineoplastic Agents: chemistry
%K Polyphenols
%K Antimicrobial effects (Other)
%K Chitosan nanoparticles (Other)
%K Cytotoxicity (Other)
%K DNA methylation (Other)
%K Tannic acid (Other)
%K Chitosan (NLM Chemicals)
%K Tannins (NLM Chemicals)
%K Anti-Infective Agents (NLM Chemicals)
%K Antineoplastic Agents (NLM Chemicals)
%K tannic acid (NLM Chemicals)
%K Polyphenols (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39638815
%R 10.1038/s41598-024-80771-x
%U https://inrepo02.dkfz.de/record/294828