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000294828 037__ $$aDKFZ-2024-02539
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000294828 1001_ $$aRashidipour, Marzieh$$b0
000294828 245__ $$aAntimicrobial activity and cytotoxic and epigenetic effects of tannic acid-loaded chitosan nanoparticles.
000294828 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2024
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000294828 520__ $$aTannic acid (TA) is a potent antitumor agent, but its low bioavailability and absorption limit its use. In this study, it was loaded into chitosan-based nanoparticles (Chi-NPs) to overcome these limitations and to improve its antimicrobial and anticancer activities. TA-loaded Chi-NPs (Chi-TA-NPs) were synthesized using the ionic gelation method and physicochemically characterized by FE-SEM, FTIR, XRD, PDI, DLS, and zeta potential analysis. Additionally, the antimicrobial activity of Chi-TA-NPs against two G+ bacterial strains, two G- bacterial strains, and a fungal strain (Candida albicans) was investigated using the microbroth dilution method. MTT assay was used to examine the cytotoxic effects of Chi-TA-NPs on HepG2 cells. The expression of DNA methyltransferase 1 (DNMT1), DNMT3A, and DNMT3B was examined in HepG2 cells using RT-qPCR. The amount of 5-methylcytosine in the HepG2 cell-derived genomic DNA was measured using ELISA. FE-SEM micrographs showed the loading of TA into the chitosan-based formulation. The peaks detected in the XRD and FTIR analyses confirmed the formation of the Chi-TA-NPs. The PDI value (0.247 ± 0.03), size (567.0 ± 25.84 nm), and zeta potential (17.0 ± 5.86 mV) confirmed the relative stability of Chi-TA-NPs. A constant release profile in line with the Korsmeyer-Peppas model was detected for Chi-TA-NPs, such that approximately 44% of TA was released after 300 min. In addition, Chi-TA-NPs exhibited effective antimicrobial activity against the studied microbial strains, as manifested by MIC values ranging from 250 to 1000 µg/mL. Chi-TA-NPs induced cytotoxicity in liver tumor cell line, with an IC50 value of 500 µg/mL. Furthermore, Chi-TA-NPs considerably decreased the expression of DNMT1 (2.52-fold; p = 0.01), DNMT3A (2.96-fold; p = 0.004), and DNMT3B (2.94-fold; p < 0.0001). However, 5-methylcytosine levels in HepG2 cells were unaffected by Chi-TA-NPs treatment (p = 0.62). Finally, the antimicrobial, cytotoxic, and epigenetic effects of Chi-TA-NPs were more pronounced than those of free TA and the unloaded Chi-NPs. In conclusion, Chi-TA-NPs exhibit promising potential for reducing microbial growth and promoting cytotoxicity in liver cancer cells.
000294828 536__ $$0G:(DE-HGF)POF4-312$$a312 - Funktionelle und strukturelle Genomforschung (POF4-312)$$cPOF4-312$$fPOF IV$$x0
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000294828 650_7 $$2Other$$aAntimicrobial effects
000294828 650_7 $$2Other$$aChitosan nanoparticles
000294828 650_7 $$2Other$$aCytotoxicity
000294828 650_7 $$2Other$$aDNA methylation
000294828 650_7 $$2Other$$aTannic acid
000294828 650_7 $$09012-76-4$$2NLM Chemicals$$aChitosan
000294828 650_7 $$2NLM Chemicals$$aTannins
000294828 650_7 $$2NLM Chemicals$$aAnti-Infective Agents
000294828 650_7 $$2NLM Chemicals$$aAntineoplastic Agents
000294828 650_7 $$2NLM Chemicals$$atannic acid
000294828 650_7 $$2NLM Chemicals$$aPolyphenols
000294828 650_2 $$2MeSH$$aChitosan: chemistry
000294828 650_2 $$2MeSH$$aChitosan: pharmacology
000294828 650_2 $$2MeSH$$aHumans
000294828 650_2 $$2MeSH$$aTannins: chemistry
000294828 650_2 $$2MeSH$$aTannins: pharmacology
000294828 650_2 $$2MeSH$$aNanoparticles: chemistry
000294828 650_2 $$2MeSH$$aHep G2 Cells
000294828 650_2 $$2MeSH$$aAnti-Infective Agents: pharmacology
000294828 650_2 $$2MeSH$$aAnti-Infective Agents: chemistry
000294828 650_2 $$2MeSH$$aEpigenesis, Genetic: drug effects
000294828 650_2 $$2MeSH$$aCandida albicans: drug effects
000294828 650_2 $$2MeSH$$aMicrobial Sensitivity Tests
000294828 650_2 $$2MeSH$$aAntineoplastic Agents: pharmacology
000294828 650_2 $$2MeSH$$aAntineoplastic Agents: chemistry
000294828 650_2 $$2MeSH$$aPolyphenols
000294828 7001_ $$aAbbaszadeh, Saber$$b1
000294828 7001_ $$aBirjandi, Mehdi$$b2
000294828 7001_ $$aPajouhi, Naser$$b3
000294828 7001_ $$aAhmadi Somaghian, Shahram$$b4
000294828 7001_ $$aGoudarzi, Gholamreza$$b5
000294828 7001_ $$0P:(DE-He78)f73ee1971b407b6179d3a0331f351355$$aShahryarhesami, Soroosh$$b6
000294828 7001_ $$aMoradi Sarabi, Mostafa$$b7
000294828 7001_ $$aBabaeenezhad, Esmaeel$$b8
000294828 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-024-80771-x$$gVol. 14, no. 1, p. 30405$$n1$$p30405$$tScientific reports$$v14$$x2045-2322$$y2024
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