TY  - JOUR
AU  - Rashidipour, Marzieh
AU  - Abbaszadeh, Saber
AU  - Birjandi, Mehdi
AU  - Pajouhi, Naser
AU  - Ahmadi Somaghian, Shahram
AU  - Goudarzi, Gholamreza
AU  - Shahryarhesami, Soroosh
AU  - Moradi Sarabi, Mostafa
AU  - Babaeenezhad, Esmaeel
TI  - Antimicrobial activity and cytotoxic and epigenetic effects of tannic acid-loaded chitosan nanoparticles.
JO  - Scientific reports
VL  - 14
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2024-02539
SP  - 30405
PY  - 2024
AB  - Tannic acid (TA) is a potent antitumor agent, but its low bioavailability and absorption limit its use. In this study, it was loaded into chitosan-based nanoparticles (Chi-NPs) to overcome these limitations and to improve its antimicrobial and anticancer activities. TA-loaded Chi-NPs (Chi-TA-NPs) were synthesized using the ionic gelation method and physicochemically characterized by FE-SEM, FTIR, XRD, PDI, DLS, and zeta potential analysis. Additionally, the antimicrobial activity of Chi-TA-NPs against two G+ bacterial strains, two G- bacterial strains, and a fungal strain (Candida albicans) was investigated using the microbroth dilution method. MTT assay was used to examine the cytotoxic effects of Chi-TA-NPs on HepG2 cells. The expression of DNA methyltransferase 1 (DNMT1), DNMT3A, and DNMT3B was examined in HepG2 cells using RT-qPCR. The amount of 5-methylcytosine in the HepG2 cell-derived genomic DNA was measured using ELISA. FE-SEM micrographs showed the loading of TA into the chitosan-based formulation. The peaks detected in the XRD and FTIR analyses confirmed the formation of the Chi-TA-NPs. The PDI value (0.247 ± 0.03), size (567.0 ± 25.84 nm), and zeta potential (17.0 ± 5.86 mV) confirmed the relative stability of Chi-TA-NPs. A constant release profile in line with the Korsmeyer-Peppas model was detected for Chi-TA-NPs, such that approximately 44
KW  - Chitosan: chemistry
KW  - Chitosan: pharmacology
KW  - Humans
KW  - Tannins: chemistry
KW  - Tannins: pharmacology
KW  - Nanoparticles: chemistry
KW  - Hep G2 Cells
KW  - Anti-Infective Agents: pharmacology
KW  - Anti-Infective Agents: chemistry
KW  - Epigenesis, Genetic: drug effects
KW  - Candida albicans: drug effects
KW  - Microbial Sensitivity Tests
KW  - Antineoplastic Agents: pharmacology
KW  - Antineoplastic Agents: chemistry
KW  - Polyphenols
KW  - Antimicrobial effects (Other)
KW  - Chitosan nanoparticles (Other)
KW  - Cytotoxicity (Other)
KW  - DNA methylation (Other)
KW  - Tannic acid (Other)
KW  - Chitosan (NLM Chemicals)
KW  - Tannins (NLM Chemicals)
KW  - Anti-Infective Agents (NLM Chemicals)
KW  - Antineoplastic Agents (NLM Chemicals)
KW  - tannic acid (NLM Chemicals)
KW  - Polyphenols (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:39638815
DO  - DOI:10.1038/s41598-024-80771-x
UR  - https://inrepo02.dkfz.de/record/294828
ER  -