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000294898 005__ 20241210182916.0
000294898 0247_ $$2URN$$ahttps://katalog.ub.uni-heidelberg.de/titel/69220146
000294898 037__ $$aDKFZ-2024-02608
000294898 041__ $$aEnglish
000294898 1001_ $$0P:(DE-He78)dff09d7cc9f06d6890e2ab76c1189ac6$$aCasati, Beatrice$$b0$$gfemale$$udkfz
000294898 245__ $$aDiagnostic and Therapeutic Applications of Programmable RNA-guided Technologies in Infection and Cancer$$f2020-05-01 - 2024-03-08
000294898 260__ $$aHeidelberg$$bUniversity of Heidelberg$$c2024
000294898 300__ $$a142
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000294898 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1733842465_7696
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000294898 502__ $$aDissertation, Universität Heidelberg, 2024$$bDissertation$$cUniversität Heidelberg$$d2024$$gFakultät für Biowissenschaften$$o2024-03-08
000294898 520__ $$aProgrammable RNA-guided technologies have sparked a revolution in both basic and translational research in the natural sciences. The discovery and development of the RNA-guided CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 system has made genome editing more accessible and broadly applicable. Its applications include, among others, characterization of gene functions, identification of disease-associated genes, and development of novel gene editing therapies. The sequence-specificity of the CRISPR-Cas system has also proven to be an invaluable tool in molecular diagnostics, where it allows the detection of specific bacterial or viral sequences during an infection. CRISPR-based diagnostic (CRISPR-Dx) technologies have been promptly applied to detect SARS-CoV-2 during the COVID-19 pandemic. In Chapter 1, I describe my contribution to this field by reporting the step-by-step optimization of a sensitive, rapid, and adaptable COVID-19 diagnostic test called ADESSO (Accurate Detection of Evolving SARS-CoV-2 through SHERLOCK Optimization) for the detection of SARS-CoV-2 and its variants.Other programmable RNA-guided technologies exploit the activity of the Adenosine Deaminase Acting on RNA (ADAR) enzymes. ADARs can be recruited by a guide RNA (gRNA) to a desired RNA sequence and induce specific adenosine to inosine (A-to-I) nucleotide changes. In Chapter 2, I describe an application of targeted ADAR-mediated RNA editing to regulate the immunogenicity of an epitope. The work described here paves the way for its application as a strategy to generate cancer neoepitopes in tumors to increase their immunogenicity and favor responsiveness to immunotherapy.
000294898 536__ $$0G:(DE-HGF)POF4-314$$a314 - Immunologie und Krebs (POF4-314)$$cPOF4-314$$fPOF IV$$x0
000294898 536__ $$0G:(GEPRIS)439669440$$aDFG project G:(GEPRIS)439669440 - TRR 319: RMaP: RNA Modifikation und Prozessierung (439669440)$$c439669440$$x1
000294898 8564_ $$uhttps://katalog.ub.uni-heidelberg.de/titel/69220146
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000294898 9141_ $$y2024
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