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@PHDTHESIS{Casati:294898,
      author       = {B. Casati$^*$},
      title        = {{D}iagnostic and {T}herapeutic {A}pplications of
                      {P}rogrammable {RNA}-guided {T}echnologies in {I}nfection
                      and {C}ancer},
      school       = {Universität Heidelberg},
      type         = {Dissertation},
      address      = {Heidelberg},
      publisher    = {University of Heidelberg},
      reportid     = {DKFZ-2024-02608},
      pages        = {142},
      year         = {2024},
      note         = {Dissertation, Universität Heidelberg, 2024},
      abstract     = {Programmable RNA-guided technologies have sparked a
                      revolution in both basic and translational research in the
                      natural sciences. The discovery and development of the
                      RNA-guided CRISPR (Clustered Regularly Interspaced Short
                      Palindromic Repeats)-Cas9 system has made genome editing
                      more accessible and broadly applicable. Its applications
                      include, among others, characterization of gene functions,
                      identification of disease-associated genes, and development
                      of novel gene editing therapies. The sequence-specificity of
                      the CRISPR-Cas system has also proven to be an invaluable
                      tool in molecular diagnostics, where it allows the detection
                      of specific bacterial or viral sequences during an
                      infection. CRISPR-based diagnostic (CRISPR-Dx) technologies
                      have been promptly applied to detect SARS-CoV-2 during the
                      COVID-19 pandemic. In Chapter 1, I describe my contribution
                      to this field by reporting the step-by-step optimization of
                      a sensitive, rapid, and adaptable COVID-19 diagnostic test
                      called ADESSO (Accurate Detection of Evolving SARS-CoV-2
                      through SHERLOCK Optimization) for the detection of
                      SARS-CoV-2 and its variants.Other programmable RNA-guided
                      technologies exploit the activity of the Adenosine Deaminase
                      Acting on RNA (ADAR) enzymes. ADARs can be recruited by a
                      guide RNA (gRNA) to a desired RNA sequence and induce
                      specific adenosine to inosine (A-to-I) nucleotide changes.
                      In Chapter 2, I describe an application of targeted
                      ADAR-mediated RNA editing to regulate the immunogenicity of
                      an epitope. The work described here paves the way for its
                      application as a strategy to generate cancer neoepitopes in
                      tumors to increase their immunogenicity and favor
                      responsiveness to immunotherapy.},
      cin          = {D150},
      cid          = {I:(DE-He78)D150-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314) / DFG project
                      G:(GEPRIS)439669440 - TRR 319: RMaP: RNA Modifikation und
                      Prozessierung (439669440)},
      pid          = {G:(DE-HGF)POF4-314 / G:(GEPRIS)439669440},
      typ          = {PUB:(DE-HGF)11},
      urn          = {https://katalog.ub.uni-heidelberg.de/titel/69220146},
      url          = {https://inrepo02.dkfz.de/record/294898},
}