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001     294898
005     20241210182916.0
024 7 _ |a https://katalog.ub.uni-heidelberg.de/titel/69220146
|2 URN
037 _ _ |a DKFZ-2024-02608
041 _ _ |a English
100 1 _ |a Casati, Beatrice
|0 P:(DE-He78)dff09d7cc9f06d6890e2ab76c1189ac6
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245 _ _ |a Diagnostic and Therapeutic Applications of Programmable RNA-guided Technologies in Infection and Cancer
|f 2020-05-01 - 2024-03-08
260 _ _ |a Heidelberg
|c 2024
|b University of Heidelberg
300 _ _ |a 142
336 7 _ |a Output Types/Dissertation
|2 DataCite
336 7 _ |a DISSERTATION
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336 7 _ |a PHDTHESIS
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336 7 _ |a Thesis
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336 7 _ |a Dissertation / PhD Thesis
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336 7 _ |a doctoralThesis
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502 _ _ |a Dissertation, Universität Heidelberg, 2024
|c Universität Heidelberg
|b Dissertation
|d 2024
|g Fakultät für Biowissenschaften
|o 2024-03-08
520 _ _ |a Programmable RNA-guided technologies have sparked a revolution in both basic and translational research in the natural sciences. The discovery and development of the RNA-guided CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 system has made genome editing more accessible and broadly applicable. Its applications include, among others, characterization of gene functions, identification of disease-associated genes, and development of novel gene editing therapies. The sequence-specificity of the CRISPR-Cas system has also proven to be an invaluable tool in molecular diagnostics, where it allows the detection of specific bacterial or viral sequences during an infection. CRISPR-based diagnostic (CRISPR-Dx) technologies have been promptly applied to detect SARS-CoV-2 during the COVID-19 pandemic. In Chapter 1, I describe my contribution to this field by reporting the step-by-step optimization of a sensitive, rapid, and adaptable COVID-19 diagnostic test called ADESSO (Accurate Detection of Evolving SARS-CoV-2 through SHERLOCK Optimization) for the detection of SARS-CoV-2 and its variants.Other programmable RNA-guided technologies exploit the activity of the Adenosine Deaminase Acting on RNA (ADAR) enzymes. ADARs can be recruited by a guide RNA (gRNA) to a desired RNA sequence and induce specific adenosine to inosine (A-to-I) nucleotide changes. In Chapter 2, I describe an application of targeted ADAR-mediated RNA editing to regulate the immunogenicity of an epitope. The work described here paves the way for its application as a strategy to generate cancer neoepitopes in tumors to increase their immunogenicity and favor responsiveness to immunotherapy.
536 _ _ |a 314 - Immunologie und Krebs (POF4-314)
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|c POF4-314
|f POF IV
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536 _ _ |a DFG project G:(GEPRIS)439669440 - TRR 319: RMaP: RNA Modifikation und Prozessierung (439669440)
|0 G:(GEPRIS)439669440
|c 439669440
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856 4 _ |u https://katalog.ub.uni-heidelberg.de/titel/69220146
909 C O |o oai:inrepo02.dkfz.de:294898
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910 1 _ |a Deutsches Krebsforschungszentrum
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914 1 _ |y 2024
920 1 _ |0 I:(DE-He78)D150-20160331
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980 _ _ |a phd
980 _ _ |a VDB
980 _ _ |a I:(DE-He78)D150-20160331
980 _ _ |a UNRESTRICTED

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