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@ARTICLE{Gnther:296018,
author = {M. Günther$^*$ and J. Sticht and C. Freund and T.
Höfer$^*$},
title = {{A}ntigen presentation by {MHC}-{II} is shaped by
competitive and cooperative allosteric mechanisms of peptide
exchange.},
journal = {Structure},
volume = {33},
number = {2},
issn = {0969-2126},
address = {Cambridge, Mass.},
publisher = {Cell Press},
reportid = {DKFZ-2024-02761},
pages = {389-400.e13},
year = {2025},
note = {#EA:B086#LA:B086# / 2025 Feb 6;33(2):389-400.e13},
abstract = {Major histocompatibility complex class II (MHC-II) presents
antigens to T helper cells. The spectrum of presented
peptides is regulated by the exchange catalyst human
leukocyte antigen DM (HLA-DM), which dissociates
peptide-MHC-II complexes in the endosome. How susceptible a
peptide is to HLA-DM is mechanistically not understood.
Here, we present a data-driven mathematical model for the
conformational landscape of MHC-II that explains the wide
range of measured HLA-DM susceptibilities and predicts why
some peptides are largely HLA-DM-resistant. We find that the
conformational plasticity of MHC-II mediates both allosteric
competition and cooperation between peptide and HLA-DM.
Competition causes HLA-DM susceptibility to be proportional
to the intrinsic peptide off-rate. Remarkably, diverse
MHC-II allotypes with conserved HLA-DM interactions show a
universal linear susceptibility function. However,
HLA-DM-resistant peptides deviate from this susceptibility
function; we predict resistance to be caused by fast peptide
association with MHC-II. Thus, our study provides
quantitative insight into peptide and MHC-II allotype
parameters that shape class-II antigen presentation.},
keywords = {HLA-DM (Other) / HLA-DM susceptibility (Other) / MHC
class-II (Other) / antigen presentation (Other) /
immunopeptidome prediction (Other) / mathematical model
(Other)},
cin = {B086},
ddc = {540},
cid = {I:(DE-He78)B086-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39708815},
doi = {10.1016/j.str.2024.11.014},
url = {https://inrepo02.dkfz.de/record/296018},
}