TY  - JOUR
AU  - Xu, Jinyun
AU  - Koch, Jonas
AU  - Schmidt, Claudia
AU  - Nientiedt, Malin
AU  - Neuberger, Manuel
AU  - Erben, Philipp
AU  - Michel, Maurice Stephan
AU  - Rodríguez-Paredes, Manuel
AU  - Lyko, Frank
TI  - Loss of YTHDC1 m6A reading function promotes invasiveness in urothelial carcinoma of the bladder.
JO  - Experimental & molecular medicine
VL  - 57
IS  - 1
SN  - 1226-3613
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2025-00031
SP  - 118-130
PY  - 2025
N1  - DKFZ-ZMBH Alliance / #EA:A130#LA:A130# / 2025 Feb;57(1):118-130
AB  - Bladder cancer poses significant clinical challenges due to its high metastatic potential and poor prognosis, especially when it progresses to muscle-invasive stages. Here, we show that the m6A reader YTHDC1 is downregulated in muscle-invasive bladder cancer and is negatively correlated with the expression of epithelial‒mesenchymal transition genes. The functional inhibition or depletion of YTHDC1 increased the migration and invasion of urothelial cells. Integrative analysis of multimodal sequencing datasets provided detailed insights into the molecular mechanisms mediating YTHDC1-dependent phenotypes and identified SMAD6 as a key transcript involved in the invasiveness of urothelial carcinoma of the bladder. Notably, SMAD6 mRNA colocalized less with YTHDC1 in tumoral tissues than in paratumoral tissues, indicating disrupted binding during cancer progression. Our findings establish YTHDC1-dependent m6A reading as a critical epitranscriptomic mechanism regulating bladder cancer invasiveness and provide a paradigm for the epitranscriptomic deregulation of cancer-associated networks.
LB  - PUB:(DE-HGF)16
C6  - pmid:39741187
DO  - DOI:10.1038/s12276-024-01377-x
UR  - https://inrepo02.dkfz.de/record/296083
ER  -