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@ARTICLE{Meurs:296113,
      author       = {R. Meurs and M. De Matos and A. Bothe and N. Guex and T.
                      Weber$^*$ and A. Teleman$^*$ and N. Ban and D. Gatfield},
      title        = {{MCTS}2 and distinct e{IF}2{D} roles in u{ORF}-dependent
                      translation regulation revealed by in vitro re-initiation
                      assays.},
      journal      = {The EMBO journal},
      volume       = {44},
      number       = {3},
      issn         = {0261-4189},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {DKFZ-2025-00043},
      pages        = {854-876},
      year         = {2025},
      note         = {2025 Feb;44(3):854-876},
      abstract     = {Ribosomes scanning from the mRNA 5' cap to the start codon
                      may initiate at upstream open reading frames (uORFs),
                      decreasing protein biosynthesis. Termination at a uORF can
                      lead to re-initiation, where 40S subunits resume scanning
                      and initiate another translation event downstream. The
                      noncanonical translation factors MCTS1-DENR participate in
                      re-initiation at specific uORFs, but knowledge of other
                      trans-acting factors or uORF features influencing
                      re-initiation is limited. Here, we establish a cell-free
                      re-initiation assay using HeLa lysates to address this
                      question. Comparing in vivo and in vitro re-initiation on
                      uORF-containing reporters, we validate MCTS1-DENR-dependent
                      re-initiation in vitro. Using this system and ribosome
                      profiling in cells, we found that knockdown of the
                      MCTS1-DENR homolog eIF2D causes widespread gene deregulation
                      unrelated to uORF translation, and thus distinct to
                      MCTS1-DENR-dependent re-initiation regulation. Additionally,
                      we identified MCTS2, encoded by an Mcts1 retrogene, as a
                      DENR partner promoting re-initiation in vitro, providing a
                      plausible explanation for clinical differences associated
                      with DENR vs. MCTS1 mutations in humans.},
      keywords     = {DENR-MCTS1 (Other) / In Vitro Translation (Other) /
                      Re-Initiation (Other) / eIF2D (Other) / uORF (Other)},
      cin          = {B140},
      ddc          = {570},
      cid          = {I:(DE-He78)B140-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39748120},
      doi          = {10.1038/s44318-024-00347-3},
      url          = {https://inrepo02.dkfz.de/record/296113},
}