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000296133 1001_ $$0P:(DE-He78)50679112f0fad6945f95f69351050e7c$$aKasan, Merve$$b0$$eFirst author$$udkfz
000296133 245__ $$aGenomic and phenotypic stability of fusion-driven pediatric sarcoma cell lines.
000296133 260__ $$a[London]$$bNature Publishing Group UK$$c2025
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000296133 520__ $$aHuman cancer cell lines are the mainstay of cancer research. Recent reports showed that highly mutated adult carcinoma cell lines (mainly HeLa and MCF-7) present striking diversity across laboratories and that long-term continuous culturing results in genomic/transcriptomic heterogeneity with strong phenotypical implications. Here, we hypothesize that oligomutated pediatric sarcoma cell lines mainly driven by a fusion transcription factor, such as Ewing sarcoma (EwS), are genetically and phenotypically more stable than the previously investigated adult carcinoma cell lines. A comprehensive molecular and phenotypic characterization of multiple EwS cell line strains, together with a simultaneous analysis during 12 months of continuous cell culture show that fusion-driven pediatric sarcoma cell line strains are genomically more stable than adult carcinoma strains, display remarkably stable and homogenous transcriptomes, and exhibit uniform and stable drug response. Additionally, the analysis of multiple EwS cell lines subjected to long-term continuous culture reveals that variable degrees of genomic/transcriptomic/phenotypic changes among fusion-driven cell lines, further exemplifying that the potential for reproducibility of in vitro scientific results may be rather understood as a spectrum, even within the same tumor entity.
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000296133 650_7 $$2NLM Chemicals$$aOncogene Proteins, Fusion
000296133 650_7 $$2NLM Chemicals$$aRNA-Binding Protein EWS
000296133 650_2 $$2MeSH$$aHumans
000296133 650_2 $$2MeSH$$aSarcoma, Ewing: genetics
000296133 650_2 $$2MeSH$$aSarcoma, Ewing: pathology
000296133 650_2 $$2MeSH$$aCell Line, Tumor
000296133 650_2 $$2MeSH$$aPhenotype
000296133 650_2 $$2MeSH$$aOncogene Proteins, Fusion: genetics
000296133 650_2 $$2MeSH$$aOncogene Proteins, Fusion: metabolism
000296133 650_2 $$2MeSH$$aRNA-Binding Protein EWS: genetics
000296133 650_2 $$2MeSH$$aRNA-Binding Protein EWS: metabolism
000296133 650_2 $$2MeSH$$aTranscriptome
000296133 650_2 $$2MeSH$$aGene Expression Regulation, Neoplastic
000296133 650_2 $$2MeSH$$aChild
000296133 650_2 $$2MeSH$$aGenomic Instability: genetics
000296133 650_2 $$2MeSH$$aGenomics: methods
000296133 650_2 $$2MeSH$$aSarcoma: genetics
000296133 650_2 $$2MeSH$$aSarcoma: pathology
000296133 650_2 $$2MeSH$$aMutation
000296133 7001_ $$0P:(DE-He78)824510670ec489266e6e294c4f8b0e6c$$aGeyer, Florian$$b1$$udkfz
000296133 7001_ $$0P:(DE-He78)a804dfb61b29a00e81bb457c172efce3$$aSiebenlist, Jana$$b2$$udkfz
000296133 7001_ $$0P:(DE-He78)45440b44791309bd4b7dbb4f73333f9b$$aSill, Martin$$b3$$udkfz
000296133 7001_ $$00000-0002-2292-5982$$aÖllinger, Rupert$$b4
000296133 7001_ $$0P:(DE-He78)40245f2168b87c014e445cabf610fd46$$aFaehling, Tobias$$b5$$udkfz
000296133 7001_ $$00000-0001-8400-046X$$ade Álava, Enrique$$b6
000296133 7001_ $$aSurdez, Didier$$b7
000296133 7001_ $$00000-0002-5435-7860$$aDirksen, Uta$$b8
000296133 7001_ $$0P:(DE-He78)908367a659dea9e28dac34592b3c46e5$$aOehme, Ina$$b9$$udkfz
000296133 7001_ $$00000-0001-6114-9499$$aScotlandi, Katia$$b10
000296133 7001_ $$00000-0002-8730-2276$$aDelattre, Olivier$$b11
000296133 7001_ $$aMüller-Nurasyid, Martina$$b12
000296133 7001_ $$0P:(DE-He78)340f7c2dcaedeae68e4a62c281c7350b$$aRad, Roland$$b13$$udkfz
000296133 7001_ $$aStrauch, Konstantin$$b14
000296133 7001_ $$0P:(DE-He78)7a590ab95c6f7ba52880452da78ecd6c$$aGrünewald, Thomas$$b15$$udkfz
000296133 7001_ $$00000-0002-0246-7179$$aCidre-Aranaz, Florencia$$b16$$eLast author
000296133 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-024-55340-5$$gVol. 16, no. 1, p. 380$$n1$$p380$$tNature Communications$$v16$$x2041-1723$$y2025
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