% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kasan:296133,
      author       = {M. Kasan$^*$ and F. Geyer$^*$ and J. Siebenlist$^*$ and M.
                      Sill$^*$ and R. Öllinger and T. Faehling$^*$ and E. de
                      Álava and D. Surdez and U. Dirksen and I. Oehme$^*$ and K.
                      Scotlandi and O. Delattre and M. Müller-Nurasyid and R.
                      Rad$^*$ and K. Strauch and T. Grünewald$^*$ and F.
                      Cidre-Aranaz$^*$},
      title        = {{G}enomic and phenotypic stability of fusion-driven
                      pediatric sarcoma cell lines.},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {DKFZ-2025-00063},
      pages        = {380},
      year         = {2025},
      note         = {#EA:B410#LA:B410#},
      abstract     = {Human cancer cell lines are the mainstay of cancer
                      research. Recent reports showed that highly mutated adult
                      carcinoma cell lines (mainly HeLa and MCF-7) present
                      striking diversity across laboratories and that long-term
                      continuous culturing results in genomic/transcriptomic
                      heterogeneity with strong phenotypical implications. Here,
                      we hypothesize that oligomutated pediatric sarcoma cell
                      lines mainly driven by a fusion transcription factor, such
                      as Ewing sarcoma (EwS), are genetically and phenotypically
                      more stable than the previously investigated adult carcinoma
                      cell lines. A comprehensive molecular and phenotypic
                      characterization of multiple EwS cell line strains, together
                      with a simultaneous analysis during 12 months of continuous
                      cell culture show that fusion-driven pediatric sarcoma cell
                      line strains are genomically more stable than adult
                      carcinoma strains, display remarkably stable and homogenous
                      transcriptomes, and exhibit uniform and stable drug
                      response. Additionally, the analysis of multiple EwS cell
                      lines subjected to long-term continuous culture reveals that
                      variable degrees of genomic/transcriptomic/phenotypic
                      changes among fusion-driven cell lines, further exemplifying
                      that the potential for reproducibility of in vitro
                      scientific results may be rather understood as a spectrum,
                      even within the same tumor entity.},
      keywords     = {Humans / Sarcoma, Ewing: genetics / Sarcoma, Ewing:
                      pathology / Cell Line, Tumor / Phenotype / Oncogene
                      Proteins, Fusion: genetics / Oncogene Proteins, Fusion:
                      metabolism / RNA-Binding Protein EWS: genetics / RNA-Binding
                      Protein EWS: metabolism / Transcriptome / Gene Expression
                      Regulation, Neoplastic / Child / Genomic Instability:
                      genetics / Genomics: methods / Sarcoma: genetics / Sarcoma:
                      pathology / Mutation / Oncogene Proteins, Fusion (NLM
                      Chemicals) / RNA-Binding Protein EWS (NLM Chemicals)},
      cin          = {B410 / HD01 / B062 / B310 / MU01},
      ddc          = {500},
      cid          = {I:(DE-He78)B410-20160331 / I:(DE-He78)HD01-20160331 /
                      I:(DE-He78)B062-20160331 / I:(DE-He78)B310-20160331 /
                      I:(DE-He78)MU01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39753532},
      pmc          = {pmc:PMC11699042},
      doi          = {10.1038/s41467-024-55340-5},
      url          = {https://inrepo02.dkfz.de/record/296133},
}