% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Wang:298179,
      author       = {H. Wang$^*$ and A. A. Syed$^*$ and J. Krijgsveld$^*$ and G.
                      Sigismondo$^*$},
      title        = {{I}solation of proteins on chromatin (i{POC}) reveals
                      signaling pathway-dependent alterations in the {DNA}-bound
                      proteome.},
      journal      = {Molecular $\&$ cellular proteomics},
      volume       = {24},
      number       = {3},
      issn         = {1535-9476},
      address      = {Bethesda, Md.},
      publisher    = {The American Society for Biochemistry and Molecular
                      Biology},
      reportid     = {DKFZ-2025-00198},
      pages        = {100908},
      year         = {2025},
      note         = {#EA:B230#LA:B230# / Volume 24, Issue 3, March 2025, 100908},
      abstract     = {Signaling pathways often convergence on transcription
                      factors (TFs) and other DNA-binding proteins (DBPs) that
                      regulate chromatin structure and gene expression, thereby
                      governing a broad range of essential cellular functions.
                      However, the repertoire of DBPs is incompletely understood
                      even for the best-characterized pathways. Here, we optimized
                      a strategy for the isolation of Proteins on Chromatin (iPOC)
                      exploiting tagged nucleoside analogues to label the DNA and
                      capture associated proteins, thus enabling the
                      comprehensive, sensitive, and unbiased characterization of
                      the DNA-bound proteome. We then applied iPOC to investigate
                      chromatome changes upon perturbation of the cancer-relevant
                      PI3K/AKT/mTOR pathway. Our results show distinct dynamics of
                      the DNA-bound proteome upon selective inhibition of PI3K,
                      AKT, or mTOR, and we provide evidence how this signaling
                      cascade regulates the DNA-bound status of SUZ12, thereby
                      modulating H3K27me3 levels. Collectively, iPOC is a powerful
                      approach to study the composition of the DNA-bound proteome
                      operating downstream of signaling cascades, thereby both
                      expanding our knowledge of the mechanism of action of the
                      pathway, and unveiling novel chromatin modulators that can
                      potentially be targeted pharmacologically.},
      keywords     = {DNA-binding proteins (Other) / H3K27me3 (Other) /
                      PI3K/AKT/mTOR signaling pathway (Other) / PRC2 (Other) /
                      SUZ12 (Other) / cancer (Other) / chromatin (Other) /
                      chromatin dynamics (Other) / pathway inhibition (Other) /
                      polycomb (Other)},
      cin          = {B230},
      ddc          = {610},
      cid          = {I:(DE-He78)B230-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39842777},
      doi          = {10.1016/j.mcpro.2025.100908},
      url          = {https://inrepo02.dkfz.de/record/298179},
}