Home > Publications database > Isolation of proteins on chromatin (iPOC) reveals signaling pathway-dependent alterations in the DNA-bound proteome. > print

001     298179
005     20250508113028.0
024 7 _ |a 10.1016/j.mcpro.2025.100908
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037 _ _ |a DKFZ-2025-00198
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Wang, Huiyu
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245 _ _ |a Isolation of proteins on chromatin (iPOC) reveals signaling pathway-dependent alterations in the DNA-bound proteome.
260 _ _ |a Bethesda, Md.
|c 2025
|b The American Society for Biochemistry and Molecular Biology
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500 _ _ |a #EA:B230#LA:B230# / Volume 24, Issue 3, March 2025, 100908
520 _ _ |a Signaling pathways often convergence on transcription factors (TFs) and other DNA-binding proteins (DBPs) that regulate chromatin structure and gene expression, thereby governing a broad range of essential cellular functions. However, the repertoire of DBPs is incompletely understood even for the best-characterized pathways. Here, we optimized a strategy for the isolation of Proteins on Chromatin (iPOC) exploiting tagged nucleoside analogues to label the DNA and capture associated proteins, thus enabling the comprehensive, sensitive, and unbiased characterization of the DNA-bound proteome. We then applied iPOC to investigate chromatome changes upon perturbation of the cancer-relevant PI3K/AKT/mTOR pathway. Our results show distinct dynamics of the DNA-bound proteome upon selective inhibition of PI3K, AKT, or mTOR, and we provide evidence how this signaling cascade regulates the DNA-bound status of SUZ12, thereby modulating H3K27me3 levels. Collectively, iPOC is a powerful approach to study the composition of the DNA-bound proteome operating downstream of signaling cascades, thereby both expanding our knowledge of the mechanism of action of the pathway, and unveiling novel chromatin modulators that can potentially be targeted pharmacologically.
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650 _ 7 |a DNA-binding proteins
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650 _ 7 |a H3K27me3
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650 _ 7 |a PI3K/AKT/mTOR signaling pathway
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650 _ 7 |a PRC2
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650 _ 7 |a SUZ12
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650 _ 7 |a cancer
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650 _ 7 |a chromatin
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650 _ 7 |a chromatin dynamics
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650 _ 7 |a pathway inhibition
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650 _ 7 |a polycomb
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700 1 _ |a Syed, Azmal Ali
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700 1 _ |a Krijgsveld, Jeroen
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700 1 _ |a Sigismondo, Gianluca
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773 _ _ |a 10.1016/j.mcpro.2025.100908
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|t Molecular & cellular proteomics
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