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@ARTICLE{Yukuyama:298186,
      author       = {M. N. Yukuyama and K. C. Fabiano and A. Inague and M. Uemi
                      and R. S. Lima and L. R. Diniz and T. E. Oliveira and T. S.
                      Iijima and H. O. F. Faria and R. S. Santos and M. F. V. Nolf
                      and A. B. Chaves-Filho$^*$ and M. Y. Yoshinaga and H. C.
                      Junqueira and P. Di Mascio and M. d. S. Baptista and S.
                      Miyamoto},
      title        = {{C}omparative study of ergosterol and 7-dehydrocholesterol
                      and their endoperoxides: {G}eneration, identification, and
                      impact in phospholipid membranes and melanoma cells.},
      journal      = {Photochemistry and photobiology},
      volume       = {101},
      number       = {4},
      issn         = {0031-8655},
      address      = {Malden, Mass.},
      publisher    = {Wiley-Blackwell},
      reportid     = {DKFZ-2025-00205},
      pages        = {960-978},
      year         = {2025},
      note         = {2025 Jul-Aug;101(4):960-978},
      abstract     = {Melanoma is an aggressive cancer that has attracted
                      attention in recent years due to its high mortality rate of
                      $80\%.$ Damage caused by oxidative stress generated by
                      radical (type I reaction) and singlet oxygen, 1O2 (type II
                      reaction) oxidative reactions may induce cancer. Thus,
                      studies that aim to unveil the mechanism that drives these
                      oxidative damage processes become relevant. Ergosterol, an
                      analogue of 7-dehydrocholesterol, important in the structure
                      of cell membranes, is widely explored in cancer treatment.
                      However, to date little is known about the impact of
                      different oxidative reactions on these sterols in melanoma
                      treatment, and conflicting results about their effectiveness
                      complicates the understanding of their role in oxidative
                      damage. Our results highlight differences among ergosterol,
                      7-dehydrocholesterol (7-DHC), and cholesterol in membrane
                      properties when subjected to distinct oxidative reactions.
                      Furthermore, we conducted a comparative study exploring the
                      mechanisms of cell damage by photodynamic treatment in A375
                      melanoma. Notably, endoperoxides from ergosterol and 7-DHC
                      generated by 1O2 showed superior efficacy in reducing the
                      viability of A375 cells compared to their precursor
                      molecules. We also describe a step-by-step process to
                      produce and identify endoperoxides derived from ergosterol
                      and 7-DHC. While further studies are needed, this work
                      provides new insights for understanding cancer cell death
                      induced by different oxidative reactions in the presence of
                      biologically relevant sterols.},
      keywords     = {7‐dehydrocholesterol (Other) / endoperoxide (Other) /
                      ergosterol (Other) / melanoma (Other) / photodynamic therapy
                      (Other) / photooxidation (Other) / singlet oxygen (Other)},
      cin          = {A410},
      ddc          = {540},
      cid          = {I:(DE-He78)A410-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39838721},
      doi          = {10.1111/php.14059},
      url          = {https://inrepo02.dkfz.de/record/298186},
}