TY  - JOUR
AU  - Ceranski, Anna Katharina
AU  - Carreño-Gonzalez, Martha J
AU  - Ehlers, Anna C
AU  - Hanssen, Kimberley M
AU  - Gmelin, Nadine
AU  - Geyer, Florian H
AU  - Kolodynska, Zuzanna
AU  - Vinca, Endrit
AU  - Faehling, Tobias
AU  - Poeller, Philipp
AU  - Ohmura, Shunya
AU  - Cidre-Aranaz, Florencia
AU  - Schulze, Almut
AU  - Grünewald, Thomas
TI  - Refined culture conditions with increased physiological relevance uncover oncogene-dependent metabolic signatures in Ewing sarcoma spheroids.
JO  - Cell reports / Methods
VL  - 5
IS  - 2
SN  - 2667-2375
CY  - Cambridge, MA
PB  - Cell Press
M1  - DKFZ-2025-00311
SP  - 100966
PY  - 2025
N1  - DKFZ-ZMBH Alliance / 2025 Feb 24;5(2):100966 / #EA:B410#LA:B410#
AB  - Ewing sarcoma (EwS) cell line culture largely relies on standard techniques, which do not recapitulate physiological conditions. Here, we report on a feasible and cost-efficient EwS cell culture technique with increased physiological relevance employing an advanced medium composition, reduced fetal calf serum, and spheroidal growth. Improved reflection of the transcriptional activity related to proliferation, hypoxia, and differentiation in EwS patient tumors was detected in EwS cells grown in this refined in vitro condition. Moreover, transcriptional signatures associated with the oncogenic activity of the EwS-specific FET::ETS fusion transcription factors in the refined culture condition were shifted from proliferative toward metabolic gene signatures. The herein-presented EwS cell culture technique with increased physiological relevance provides a broadly applicable approach for enhanced in vitro modeling relevant to advancing EwS research and the validity of experimental results.
KW  - CP: Cancer biology (Other)
KW  - CP: Metabolism (Other)
KW  - Ewing sarcoma (Other)
KW  - fetal calf serum (Other)
KW  - modeling adequacy (Other)
KW  - physiological media (Other)
KW  - refined cell culture (Other)
KW  - spherioidal growth (Other)
KW  - transcriptome (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39922188
DO  - DOI:10.1016/j.crmeth.2025.100966
UR  - https://inrepo02.dkfz.de/record/298611
ER  -