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@ARTICLE{Ceranski:298611,
      author       = {A. K. Ceranski$^*$ and M. J. Carreño-Gonzalez$^*$ and A.
                      C. Ehlers$^*$ and K. M. Hanssen$^*$ and N. Gmelin$^*$ and F.
                      H. Geyer$^*$ and Z. Kolodynska$^*$ and E. Vinca$^*$ and T.
                      Faehling$^*$ and P. Poeller$^*$ and S. Ohmura$^*$ and F.
                      Cidre-Aranaz$^*$ and A. Schulze$^*$ and T. Grünewald$^*$},
      title        = {{R}efined culture conditions with increased physiological
                      relevance uncover oncogene-dependent metabolic signatures in
                      {E}wing sarcoma spheroids.},
      journal      = {Cell reports / Methods},
      volume       = {5},
      number       = {2},
      issn         = {2667-2375},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-00311},
      pages        = {100966},
      year         = {2025},
      note         = {DKFZ-ZMBH Alliance / 2025 Feb 24;5(2):100966 /
                      #EA:B410#LA:B410#},
      abstract     = {Ewing sarcoma (EwS) cell line culture largely relies on
                      standard techniques, which do not recapitulate physiological
                      conditions. Here, we report on a feasible and cost-efficient
                      EwS cell culture technique with increased physiological
                      relevance employing an advanced medium composition, reduced
                      fetal calf serum, and spheroidal growth. Improved reflection
                      of the transcriptional activity related to proliferation,
                      hypoxia, and differentiation in EwS patient tumors was
                      detected in EwS cells grown in this refined in vitro
                      condition. Moreover, transcriptional signatures associated
                      with the oncogenic activity of the EwS-specific FET::ETS
                      fusion transcription factors in the refined culture
                      condition were shifted from proliferative toward metabolic
                      gene signatures. The herein-presented EwS cell culture
                      technique with increased physiological relevance provides a
                      broadly applicable approach for enhanced in vitro modeling
                      relevant to advancing EwS research and the validity of
                      experimental results.},
      keywords     = {CP: Cancer biology (Other) / CP: Metabolism (Other) / Ewing
                      sarcoma (Other) / fetal calf serum (Other) / modeling
                      adequacy (Other) / physiological media (Other) / refined
                      cell culture (Other) / spherioidal growth (Other) /
                      transcriptome (Other)},
      cin          = {B410 / HD01 / A410},
      ddc          = {610},
      cid          = {I:(DE-He78)B410-20160331 / I:(DE-He78)HD01-20160331 /
                      I:(DE-He78)A410-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39922188},
      doi          = {10.1016/j.crmeth.2025.100966},
      url          = {https://inrepo02.dkfz.de/record/298611},
}