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000298618 041__ $$aEnglish
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000298618 1001_ $$00000-0002-6703-7997$$aKardell, Oliver$$b0
000298618 245__ $$aMulticenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum.
000298618 260__ $$aWashington, DC$$bACS Publications$$c2025
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000298618 500__ $$aHI-TRON / 2025 Mar 7;24(3):1017-1029
000298618 520__ $$aAdvancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, and precision. In a joint effort, eight proteomics laboratories belonging to the MSCoreSys initiative including the CLINSPECT-M, MSTARS, DIASyM, and SMART-CARE consortia performed a longitudinal round-robin study to assess the analysis performance of plasma and serum as clinically relevant samples. A variety of LC-MS/MS setups including mass spectrometer models from ThermoFisher and Bruker as well as LC systems from ThermoFisher, Evosep, and Waters Corporation were used in this study. As key performance indicators, sensitivity, precision, and reproducibility were monitored over time. Protein identifications range between 300 and 400 IDs across different state-of-the-art MS instruments, with timsTOF Pro, Orbitrap Exploris 480, and Q Exactive HF-X being among the top performers. Overall, 71 proteins are reproducibly detectable in all setups in both serum and plasma samples, and 22 of these proteins are FDA-approved biomarkers, which are reproducibly quantified (CV < 20% with label-free quantification). In total, the round-robin study highlights a promising baseline for bringing MS-based measurements of serum and plasma samples closer to clinical utility.
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000298618 650_7 $$2Other$$aLC-MS/MS
000298618 650_7 $$2Other$$aR package mpwR
000298618 650_7 $$2Other$$aclinical specimen
000298618 650_7 $$2Other$$alongitudinal round-robin study
000298618 650_7 $$2Other$$aplasma
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000298618 7001_ $$aGronauer, Thomas$$b1
000298618 7001_ $$avon Toerne, Christine$$b2
000298618 7001_ $$aMerl-Pham, Juliane$$b3
000298618 7001_ $$aKönig, Ann-Christine$$b4
000298618 7001_ $$aBarth, Teresa K$$b5
000298618 7001_ $$aMergner, Julia$$b6
000298618 7001_ $$00000-0002-6131-7322$$aLudwig, Christina$$b7
000298618 7001_ $$aTüshaus, Johanna$$b8
000298618 7001_ $$aGiesbertz, Pieter$$b9
000298618 7001_ $$00000-0002-0305-3373$$aBreimann, Stephan$$b10
000298618 7001_ $$aSchweizer, Lisa$$b11
000298618 7001_ $$0P:(DE-He78)f0c2bd6811990c0fd74ffcd3f59d9712$$aMüller, Torsten$$b12
000298618 7001_ $$0P:(DE-He78)26ae2fbc5967afaee49569bc4845f65a$$aKliewer, Georg$$b13
000298618 7001_ $$00000-0002-8031-6384$$aDistler, Ute$$b14
000298618 7001_ $$0P:(DE-He78)4569ef2919d2438765ad71515f53646b$$aGómez-Zepeda, David$$b15$$udkfz
000298618 7001_ $$aPopp, Oliver$$b16
000298618 7001_ $$aQin, Di$$b17
000298618 7001_ $$aTeupser, Daniel$$b18
000298618 7001_ $$00000-0001-8597-205X$$aCox, Jürgen$$b19
000298618 7001_ $$00000-0003-2993-8249$$aImhof, Axel$$b20
000298618 7001_ $$00000-0002-9094-1677$$aKüster, Bernhard$$b21
000298618 7001_ $$aLichtenthaler, Stefan F$$b22
000298618 7001_ $$0P:(DE-He78)939d5891259c638c1ab053b1456a578c$$aKrijgsveld, Jeroen$$b23$$udkfz
000298618 7001_ $$aTenzer, Stefan$$b24
000298618 7001_ $$aMertins, Philipp$$b25
000298618 7001_ $$00000-0002-2244-5081$$aCoscia, Fabian$$b26
000298618 7001_ $$aHauck, Stefanie M$$b27
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