% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Declercq:298620,
author = {A. Declercq and R. Devreese and J. Scheid and C. Jachmann
and T. Van Den Bossche and A. Preikschat and D.
Gómez-Zepeda$^*$ and J. B. Rijal and A. Hirschler and J. R.
Krieger and T. Srikumar and G. Rosenberger and C. Martelli
and D. Trede and C. Carapito and S. Tenzer and J. S.
Walz$^*$ and S. Degroeve and R. Bouwmeester and L. Martens
and R. Gabriels},
title = {{TIMS}2{R}escore: {A} {D}ata {D}ependent
{A}cquisition-{P}arallel {A}ccumulation and {S}erial
{F}ragmentation-{O}ptimized {D}ata-{D}riven {R}escoring
{P}ipeline {B}ased on {MS}2{R}escore.},
journal = {Journal of proteome research},
volume = {24},
number = {3},
issn = {1535-3893},
address = {Washington, DC},
publisher = {ACS Publications},
reportid = {DKFZ-2025-00320},
pages = {1067-1076},
year = {2025},
note = {HI-TRON / 2025 Mar 7;24(3):1067-1076},
abstract = {The high throughput analysis of proteins with mass
spectrometry (MS) is highly valuable for understanding human
biology, discovering disease biomarkers, identifying
therapeutic targets, and exploring pathogen interactions. To
achieve these goals, specialized proteomics subfields,
including plasma proteomics, immunopeptidomics, and
metaproteomics, must tackle specific analytical challenges,
such as an increased identification ambiguity compared to
routine proteomics experiments. Technical advancements in MS
instrumentation can mitigate these issues by acquiring more
discerning information at higher sensitivity levels. This is
exemplified by the incorporation of ion mobility and
parallel accumulation and serial fragmentation (PASEF)
technologies in timsTOF instruments. In addition, AI-based
bioinformatics solutions can help overcome ambiguity issues
by integrating more data into the identification workflow.
Here, we introduce TIMS2Rescore, a data-driven rescoring
workflow optimized for DDA-PASEF data from timsTOF
instruments. This platform includes new timsTOF MS2PIP
spectrum prediction models and IM2Deep, a new deep
learning-based peptide ion mobility predictor. Furthermore,
to fully streamline data throughput, TIMS2Rescore directly
accepts Bruker raw mass spectrometry data and search results
from ProteoScape and many other search engines, including
Sage and PEAKS. We showcase TIMS2Rescore performance on
plasma proteomics, immunopeptidomics (HLA class I and II),
and metaproteomics data sets. TIMS2Rescore is open-source
and freely available at
https://github.com/compomics/tims2rescore.},
keywords = {DDA-PASEF (Other) / machine learning (Other) / mass
spectrometry (Other) / peptide identification (Other) /
proteomics (Other) / rescoring (Other) / timsTOF (Other)},
cin = {TU01 / D191},
ddc = {540},
cid = {I:(DE-He78)TU01-20160331 / I:(DE-He78)D191-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39915959},
doi = {10.1021/acs.jproteome.4c00609},
url = {https://inrepo02.dkfz.de/record/298620},
}