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@ARTICLE{Adabi:298908,
      author       = {E. Adabi and F. T. Charitidis and F. B. Thalheimer and M.
                      Guaza-Lasheras and C. Clarke and C. J. Buchholz$^*$},
      title        = {{E}nhanced conversion of {T} cells into {CAR} {T} cells by
                      modulation of the {MAPK}/{ERK} pathway.},
      journal      = {Cell reports / Medicine},
      volume       = {6},
      number       = {2},
      issn         = {2666-3791},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-00347},
      pages        = {101970},
      year         = {2025},
      note         = {2025 Feb 18;6(2):101970},
      abstract     = {Delivery of chimeric antigen receptors (CARs) to T cells is
                      usually mediated by lentiviral vectors (LVs), which can have
                      broad tropism or be T cell targeted. To better understand
                      the molecular events during CAR T cell generation, T cell
                      transduction with four different LVs is followed by
                      single-cell multi-omics analysis, distinguishing between
                      transduced T cells and T cells with vector signal but no
                      CAR. We find that only a fraction of the T cells that
                      encounter vectors convert into CAR T cells. Single-cell
                      transcriptome data reveal that interferon-stimulated genes
                      are upregulated in non-transduced cells, whereas
                      extracellular signal-regulated kinase (ERK)2 phosphatases
                      are upregulated in CAR T cells. This expression pattern is
                      evident in CAR T cells from healthy donors and patients. The
                      role of the mitogen-activated protein kinase (MAPK)/ERK
                      pathway in CAR T cell generation is confirmed by chemical
                      inhibitors. These data provide molecular insights into T
                      cell transduction with implications for improving CAR T cell
                      generation.},
      keywords     = {CAR T cells, lentiviral vector, adeno-associated vector,
                      interferon-stimulated genes, MAPK/ERK pathway, ERK2
                      inhibitors (Other)},
      cin          = {FM01},
      ddc          = {610},
      cid          = {I:(DE-He78)FM01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39938523},
      doi          = {10.1016/j.xcrm.2025.101970},
      url          = {https://inrepo02.dkfz.de/record/298908},
}