TY  - JOUR
AU  - Cibir, Zülal
AU  - Beer, Alexander
AU  - Kraus, Andreas
AU  - Pillibeit, Aleksandra
AU  - Bludau, Dana
AU  - Abdulla, Haji
AU  - Neuendorff, Nina Rosa
AU  - Sonneck, Justin
AU  - Kowitz, Lennart
AU  - Riese, Stefanie
AU  - Tuz, Ali Ata
AU  - Chen, Jianxu
AU  - Cherneha, Maxim
AU  - Beelen, Dietrich W
AU  - Reinhardt, H Christian
AU  - Gunzer, Matthias
AU  - Turki, Amin T
TI  - Risk beyond neutropenia: insights into neutrophil migration from newly diagnosed AML until late after allogeneic stem cell transplantation.
JO  - Journal of leukocyte biology
VL  - 117
IS  - 2
SN  - 0741-5400
CY  - Tokyo
PB  - Oxford University Press
M1  - DKFZ-2025-00362
SP  - qiae250
PY  - 2025
AB  - Quantification of neutrophil counts is the most relevant assessment of cellular immunity in clinical practice. Patients with neutropenia are considered at risk and are categorized according to its severity. The incidence of febrile neutropenia varies, but patients with acute myeloid leukemia are traditionally considered at high risk, especially following myelotoxic treatments. To provide additional functional parameters, we investigated the ex vivo migration properties and morphology of neutrophils in 10 patients with acute myeloid leukemia using single-cell video-microscopy and discovered, in addition to neutropenia, highly pathological neutrophil migration patterns and polarization defects in patients with untreated acute myeloid leukemia. Neutrophil speed was the most sensitive parameter and significantly lower at leukemia diagnosis (9.067 vs 15.810 µm/min, P = 0.0025) compared to healthy controls (n = 46). Hematological remission was associated with improved neutrophil migration profiles, but these ultimately normalized only after hematopoietic cell transplantation. Five patients were followed up for long-term effects of hematopoietic cell transplantation for up to 24 mo. This is the first longitudinal ex vivo neutrophil migration study in patients with acute myeloid leukemia, followed by allogeneic hematopoietic cell transplantation. It identified functional neutrophil impairments beyond routine quantitative assessments, adding to the well-known quantitative impairment of neutropenia. HCT can reestablish functional neutrophils with healthy migration profiles in these patients.
KW  - Humans
KW  - Leukemia, Myeloid, Acute: therapy
KW  - Leukemia, Myeloid, Acute: immunology
KW  - Leukemia, Myeloid, Acute: pathology
KW  - Neutrophils: immunology
KW  - Neutrophils: pathology
KW  - Male
KW  - Female
KW  - Middle Aged
KW  - Neutropenia: etiology
KW  - Neutropenia: pathology
KW  - Neutropenia: immunology
KW  - Adult
KW  - Hematopoietic Stem Cell Transplantation: adverse effects
KW  - Aged
KW  - Transplantation, Homologous: adverse effects
KW  - Cell Movement
KW  - Young Adult
KW  - acute myeloid leukemia (Other)
KW  - myeloid neoplasia (Other)
KW  - neutrophil granulocytes (Other)
KW  - transplantation (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39953806
DO  - DOI:10.1093/jleuko/qiae250
UR  - https://inrepo02.dkfz.de/record/298928
ER  -