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@ARTICLE{Lim:298936,
author = {B. Lim$^*$ and A. Kamal and B. Gomez Ramos$^*$ and J.
Adrian Segarra$^*$ and I. L. Ibarra and L. Dignas$^*$ and T.
Kindinger$^*$ and K. Volz$^*$ and M. Rahbari$^*$ and N.
Rahbari and E. Poisel$^*$ and K. Kafetzopoulou$^*$ and L.
Böse$^*$ and M. Breinig$^*$ and D. Heide$^*$ and S. U.
Gallage$^*$ and J. E. Barragan Avila$^*$ and H. Wiethoff and
I. Berest and S. Schnabellehner and M. Schneider$^*$ and J.
Becker$^*$ and D. Helm$^*$ and D. Grimm and T. Mäkinen and
D. F. Tschaharganeh$^*$ and M. Heikenwalder$^*$ and J. B.
Zaugg and M. Mall$^*$},
title = {{A}ctive repression of cell fate plasticity by {PROX}1
safeguards hepatocyte identity and prevents liver
tumorigenesis.},
journal = {Nature genetics},
volume = {57},
number = {3},
issn = {1061-4036},
address = {London},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {DKFZ-2025-00370},
pages = {668-679},
year = {2025},
note = {DKFZ-ZMBH Alliance /#EA:A340#LA:A340# / 2025
Mar;57(3):668-679},
abstract = {Cell fate plasticity enables development, yet unlocked
plasticity is a cancer hallmark. While transcription master
regulators induce lineage-specific genes to restrict
plasticity, it remains unclear whether plasticity is
actively suppressed by lineage-specific repressors. Here we
computationally predict so-called safeguard repressors for
18 cell types that block phenotypic plasticity lifelong. We
validated hepatocyte-specific candidates using
reprogramming, revealing that prospero homeobox protein 1
(PROX1) enhanced hepatocyte identity by direct repression of
alternative fate master regulators. In mice, Prox1 was
required for efficient hepatocyte regeneration after injury
and was sufficient to prevent liver tumorigenesis. In line
with patient data, Prox1 depletion caused hepatocyte fate
loss in vivo and enabled the transition of hepatocellular
carcinoma to cholangiocarcinoma. Conversely, overexpression
promoted cholangiocarcinoma to hepatocellular carcinoma
transdifferentiation. Our findings provide evidence for
PROX1 as a hepatocyte-specific safeguard and support a model
where cell-type-specific repressors actively suppress
plasticity throughout life to safeguard lineage identity and
thus prevent disease.},
cin = {A340 / F190 / D440 / W120},
ddc = {570},
cid = {I:(DE-He78)A340-20160331 / I:(DE-He78)F190-20160331 /
I:(DE-He78)D440-20160331 / I:(DE-He78)W120-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39948437},
doi = {10.1038/s41588-025-02081-w},
url = {https://inrepo02.dkfz.de/record/298936},
}