000299001 001__ 299001
000299001 005__ 20250819095805.0
000299001 0247_ $$2doi$$a10.1038/s44318-025-00370-y
000299001 0247_ $$2pmid$$apmid:39984755
000299001 0247_ $$2ISSN$$a0261-4189
000299001 0247_ $$2ISSN$$a1460-2075
000299001 0247_ $$2altmetric$$aaltmetric:174391781
000299001 037__ $$aDKFZ-2025-00411
000299001 041__ $$aEnglish
000299001 082__ $$a570
000299001 1001_ $$aSmarduch, Steve$$b0
000299001 245__ $$aA novel biosensor for the spatiotemporal analysis of STING activation during innate immune responses to dsDNA.
000299001 260__ $$a[London]$$bNature Publishing Group UK$$c2025
000299001 3367_ $$2DRIVER$$aarticle
000299001 3367_ $$2DataCite$$aOutput Types/Journal article
000299001 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1755590255_6402
000299001 3367_ $$2BibTeX$$aARTICLE
000299001 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000299001 3367_ $$00$$2EndNote$$aJournal Article
000299001 500__ $$a2025 Apr;44(7):2157-2182
000299001 520__ $$aThe cGAS-STING signalling pathway has a central role in the innate immune response to extrinsic and intrinsic sources of cytoplasmic dsDNA. At the core of this pathway is cGAS-dependent production of the intra- and extra-cellular messenger cGAMP, which activates STING and leads to IRF3-dependent expression of cytokines and interferons. Despite its relevance to viral and bacterial infections, cell death, and genome instability, the lack of specific live-cell reporters has precluded spatiotemporal analyses of cGAS-STING signalling. Here, we generate a fluorescent biosensor termed SIRF (STING-IRF3), which reports on the functional interaction between activated STING and IRF3 at the Golgi. We show that cells harbouring SIRF react in a time- and concentration-dependent manner both to STING agonists and to microenvironmental cGAMP. We demonstrate that the new biosensor is suitable for single-cell characterisation of immune responses to HSV-1 infection, mtDNA release upon apoptosis, or other sources of cytoplasmic dsDNA. Furthermore, our results indicate that STING signalling is not activated by ruptured micronuclei, suggesting that other cytosolic pattern recognition receptors underlie the interferon responses to chromosomal instability.
000299001 536__ $$0G:(DE-HGF)POF4-314$$a314 - Immunologie und Krebs (POF4-314)$$cPOF4-314$$fPOF IV$$x0
000299001 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000299001 650_7 $$2Other$$aBiosensor
000299001 650_7 $$2Other$$aInnate Immune Response
000299001 650_7 $$2Other$$aMicronuclei
000299001 650_7 $$2Other$$acGAMP
000299001 650_7 $$2Other$$acGAS-STING Signalling
000299001 7001_ $$00000-0001-7333-275X$$aMoreno-Velasquez, Sergio David$$b1
000299001 7001_ $$0P:(DE-He78)10525bf5474612b51e75b27f6e69fd71$$aIlic, Doroteja$$b2$$udkfz
000299001 7001_ $$00000-0002-0227-5900$$aDadsena, Shashank$$b3
000299001 7001_ $$aMorant, Ryan$$b4
000299001 7001_ $$aCiprinidis, Anja$$b5
000299001 7001_ $$0P:(DE-He78)5138c10dcd0901d001d67a31423402b5$$aPereira, Gislene$$b6$$udkfz
000299001 7001_ $$0P:(DE-He78)2fb2bd9048a3777dddc4cb89b115c187$$aBinder, Marco$$b7$$udkfz
000299001 7001_ $$00000-0002-3894-5945$$aGarcía-Sáez, Ana J$$b8
000299001 7001_ $$00000-0002-7694-2497$$aAcebrón, Sergio P$$b9
000299001 773__ $$0PERI:(DE-600)1467419-1$$a10.1038/s44318-025-00370-y$$n7$$p2157-2182$$tThe EMBO journal$$v44$$x0261-4189$$y2025
000299001 909CO $$ooai:inrepo02.dkfz.de:299001$$pVDB
000299001 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)10525bf5474612b51e75b27f6e69fd71$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000299001 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)5138c10dcd0901d001d67a31423402b5$$aDeutsches Krebsforschungszentrum$$b6$$kDKFZ
000299001 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)2fb2bd9048a3777dddc4cb89b115c187$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000299001 9131_ $$0G:(DE-HGF)POF4-314$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vImmunologie und Krebs$$x0
000299001 9141_ $$y2025
000299001 915__ $$0StatID:(DE-HGF)3001$$2StatID$$aDEAL Wiley$$d2024-12-30$$wger
000299001 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-04-08T07:44:01Z
000299001 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-04-08T07:44:01Z
000299001 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review, Double anonymous peer review$$d2024-04-08T07:44:01Z
000299001 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2024-04-08T07:44:01Z
000299001 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bEMBO J : 2022$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bEMBO J : 2022$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-30
000299001 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-30
000299001 9201_ $$0I:(DE-He78)D430-20160331$$kD430$$lVirus-assoziierte Karzinogenese$$x0
000299001 9201_ $$0I:(DE-He78)A180-20160331$$kA180$$lA180 Molecular Biology of Centrosomes$$x1
000299001 980__ $$ajournal
000299001 980__ $$aVDB
000299001 980__ $$aI:(DE-He78)D430-20160331
000299001 980__ $$aI:(DE-He78)A180-20160331
000299001 980__ $$aUNRESTRICTED