%0 Journal Article
%A Schwerd-Kleine, Paul
%A Würth, Roberto
%A Cheytan, Tasneem
%A Michel, Laura
%A Thewes, Verena
%A Gutjahr, Ewgenija
%A Seker-Cin, Huriye
%A Kazdal, Daniel
%A Neuberth, Sarah-Jane
%A Thiel, Vera
%A Schwickert, Jonas
%A Vorberg, Tim
%A Wischhusen, Jennifer
%A Stenzinger, Albrecht
%A Zapatka, Marc
%A Lichter, Peter
%A Schneeweiss, Andreas
%A Trumpp, Andreas
%A Sprick, Martin
%T Biopsy-derived organoids in personalised early breast cancer care: Challenges of tumour purity and normal cell overgrowth cap their practical utility.
%J International journal of cancer
%V 156
%N 11
%@ 0020-7136
%C Bognor Regis
%I Wiley-Liss
%M DKFZ-2025-00463
%P 2200-2209
%D 2025
%Z #EA:A010#LA:A010# / 2025 Jun 1;156(11):2200-2209
%X The ability to establish organoids composed exclusively of tumour rather than healthy cells is essential for their implementation into clinical practice. Organoids have recently emerged as a powerful tool to expand patient material in culture and generate modifiable 3D models derived from humans or animal models. For translational research, they enable the creation of model systems for an ever-increasing number of cell types and diseases. And in personalised medicine, they potentially allow for functional drug testing with high predictive power in certain settings. We found that using biopsy material from untreated, early-stage primary breast cancer patients poses significant challenges for consistently culturing tumour cells as organoids. Specifically, we observed frequent outgrowth of genetically normal, non-cancerous epithelial cells. We analysed >100 biopsy samples from early-stage breast cancer and present our large collection of >70 organoid lines. We also show methods of assessing successful tumour cell culture in a time, and cost-efficient manner, proving a high rate (>85
%K breast cancer (Other)
%K genomics (Other)
%K organoids (Other)
%K personalized medicine (Other)
%K quality control (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40022208
%R DOI:10.1002/ijc.35386
%U https://inrepo02.dkfz.de/record/299505