TY  - JOUR
AU  - Schwerd-Kleine, Paul
AU  - Würth, Roberto
AU  - Cheytan, Tasneem
AU  - Michel, Laura
AU  - Thewes, Verena
AU  - Gutjahr, Ewgenija
AU  - Seker-Cin, Huriye
AU  - Kazdal, Daniel
AU  - Neuberth, Sarah-Jane
AU  - Thiel, Vera
AU  - Schwickert, Jonas
AU  - Vorberg, Tim
AU  - Wischhusen, Jennifer
AU  - Stenzinger, Albrecht
AU  - Zapatka, Marc
AU  - Lichter, Peter
AU  - Schneeweiss, Andreas
AU  - Trumpp, Andreas
AU  - Sprick, Martin
TI  - Biopsy-derived organoids in personalised early breast cancer care: Challenges of tumour purity and normal cell overgrowth cap their practical utility.
JO  - International journal of cancer
VL  - 156
IS  - 11
SN  - 0020-7136
CY  - Bognor Regis
PB  - Wiley-Liss
M1  - DKFZ-2025-00463
SP  - 2200-2209
PY  - 2025
N1  - #EA:A010#LA:A010# / 2025 Jun 1;156(11):2200-2209
AB  - The ability to establish organoids composed exclusively of tumour rather than healthy cells is essential for their implementation into clinical practice. Organoids have recently emerged as a powerful tool to expand patient material in culture and generate modifiable 3D models derived from humans or animal models. For translational research, they enable the creation of model systems for an ever-increasing number of cell types and diseases. And in personalised medicine, they potentially allow for functional drug testing with high predictive power in certain settings. We found that using biopsy material from untreated, early-stage primary breast cancer patients poses significant challenges for consistently culturing tumour cells as organoids. Specifically, we observed frequent outgrowth of genetically normal, non-cancerous epithelial cells. We analysed >100 biopsy samples from early-stage breast cancer and present our large collection of >70 organoid lines. We also show methods of assessing successful tumour cell culture in a time, and cost-efficient manner, proving a high rate (>85
KW  - breast cancer (Other)
KW  - genomics (Other)
KW  - organoids (Other)
KW  - personalized medicine (Other)
KW  - quality control (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40022208
DO  - DOI:DOI:10.1002/ijc.35386
UR  - https://inrepo02.dkfz.de/record/299505
ER  -