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@ARTICLE{SchwerdKleine:299505,
      author       = {P. Schwerd-Kleine$^*$ and R. Würth$^*$ and T. Cheytan$^*$
                      and L. Michel and V. Thewes$^*$ and E. Gutjahr$^*$ and H.
                      Seker-Cin and D. Kazdal and S.-J. Neuberth$^*$ and V.
                      Thiel$^*$ and J. Schwickert$^*$ and T. Vorberg$^*$ and J.
                      Wischhusen$^*$ and A. Stenzinger and M. Zapatka$^*$ and P.
                      Lichter$^*$ and A. Schneeweiss and A. Trumpp$^*$ and M.
                      Sprick$^*$},
      title        = {{B}iopsy-derived organoids in personalised early breast
                      cancer care: {C}hallenges of tumour purity and normal cell
                      overgrowth cap their practical utility.},
      journal      = {International journal of cancer},
      volume       = {156},
      number       = {11},
      issn         = {0020-7136},
      address      = {Bognor Regis},
      publisher    = {Wiley-Liss},
      reportid     = {DKFZ-2025-00463},
      pages        = {2200-2209},
      year         = {2025},
      note         = {#EA:A010#LA:A010# / 2025 Jun 1;156(11):2200-2209},
      abstract     = {The ability to establish organoids composed exclusively of
                      tumour rather than healthy cells is essential for their
                      implementation into clinical practice. Organoids have
                      recently emerged as a powerful tool to expand patient
                      material in culture and generate modifiable 3D models
                      derived from humans or animal models. For translational
                      research, they enable the creation of model systems for an
                      ever-increasing number of cell types and diseases. And in
                      personalised medicine, they potentially allow for functional
                      drug testing with high predictive power in certain settings.
                      We found that using biopsy material from untreated,
                      early-stage primary breast cancer patients poses significant
                      challenges for consistently culturing tumour cells as
                      organoids. Specifically, we observed frequent outgrowth of
                      genetically normal, non-cancerous epithelial cells. We
                      analysed >100 biopsy samples from early-stage breast cancer
                      and present our large collection of >70 organoid lines. We
                      also show methods of assessing successful tumour cell
                      culture in a time, and cost-efficient manner, proving a high
                      rate $(>85\%)$ of normal cell overgrowth in early-stage
                      breast cancer organoids. Finally, we show a number of
                      successful attempts to culture cancer organoids from
                      mastectomy-derived tissue of advanced, metastatic breast
                      cancer. We conclude that the usefulness of organoids from
                      early breast cancer for translational research and
                      personalised medicine, especially guidance of adjuvant or
                      post-surgical maintenance therapy, is strongly limited by
                      the low success rate of culturing cancerous cells under
                      organoid conditions.},
      keywords     = {breast cancer (Other) / genomics (Other) / organoids
                      (Other) / personalized medicine (Other) / quality control
                      (Other)},
      cin          = {A010 / B060},
      ddc          = {610},
      cid          = {I:(DE-He78)A010-20160331 / I:(DE-He78)B060-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40022208},
      doi          = {DOI:10.1002/ijc.35386},
      url          = {https://inrepo02.dkfz.de/record/299505},
}