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@ARTICLE{Aivazidis:299517,
author = {A. Aivazidis and F. Memi and V. Kleshchevnikov and S. Er
and B. Clarke$^*$ and O. Stegle$^*$ and O. A. Bayraktar},
title = {{C}ell2fate infers {RNA} velocity modules to improve cell
fate prediction.},
journal = {Nature methods},
volume = {22},
number = {4},
issn = {1548-7091},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {DKFZ-2025-00475},
pages = {698-707},
year = {2025},
note = {2025 Apr;22(4):698-707},
abstract = {RNA velocity exploits the temporal information contained in
spliced and unspliced RNA counts to infer transcriptional
dynamics. Existing velocity models often rely on coarse
biophysical simplifications or numerical approximations to
solve the underlying ordinary differential equations (ODEs),
which can compromise accuracy in challenging settings, such
as complex or weak transcription rate changes across
cellular trajectories. Here we present cell2fate, a
formulation of RNA velocity based on a linearization of the
velocity ODE, which allows solving a biophysically more
accurate model in a fully Bayesian fashion. As a result,
cell2fate decomposes the RNA velocity solutions into
modules, providing a biophysical connection between RNA
velocity and statistical dimensionality reduction. We
comprehensively benchmark cell2fate in real-world settings,
demonstrating enhanced interpretability and power to
reconstruct complex dynamics and weak dynamical signals in
rare and mature cell types. Finally, we apply cell2fate to
the developing human brain, where we spatially map RNA
velocity modules onto the tissue architecture, connecting
the spatial organization of tissues with temporal dynamics
of transcription.},
cin = {B260},
ddc = {610},
cid = {I:(DE-He78)B260-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40032996},
doi = {10.1038/s41592-025-02608-3},
url = {https://inrepo02.dkfz.de/record/299517},
}