% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Wimmers:299786,
      author       = {D. G. Wimmers and K. Huebner and T. Dale and A. Papargyriou
                      and M. Reichert$^*$ and A. Hartmann and R. Schneider-Stock},
      title        = {{A} floating collagen matrix triggers ring formation and
                      stemness characteristics in human colorectal cancer
                      organoids.},
      journal      = {Pathology, research and practice},
      volume       = {269},
      issn         = {0344-0338},
      address      = {München},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2025-00541},
      pages        = {155890},
      year         = {2025},
      abstract     = {Intestinal organoids reflect the 3D structure and function
                      of their original tissues. Organoid are typically cultured
                      in Matrigel, an extracellular matrix (ECM) mimicking the
                      basement membrane, which is suitable for epithelial cells
                      but does not accurately mimic the tumour microenvironment of
                      colorectal cancer (CRC). The ECM and particularly collagen
                      type I is crucial for CRC progression and invasiveness.
                      Given that efforts to examine CRC organoid invasion in a
                      more physiologically relevant ECM have been limited, we used
                      a floating collagen type I matrix (FC) to study organoid
                      invasion in three patient-derived CRC organoid lines. In FC
                      gel, organoids contract, align, and fuse into macroscopic
                      ring structures, initiating minor branch formation and
                      invasion fronts, phenomena unique for the collagen ECM and
                      otherwise not observed in Matrigel-grown CRC organoids. In
                      contrast to Matrigel, FC organoids showed basal extrusion
                      with improper actin localization, but without change in the
                      organoid polarity. Moreover, small clusters of vital
                      invading cells were observed. Gene expression analysis
                      revealed that the organoids cultured in a FC matrix
                      presented more epithelial and stem cell-like
                      characteristics. This novel technique of cultivating CRC
                      organoids in a FC matrix represents an in-vitro model for
                      studying cancer organization and matrix remodelling with
                      increased organoid stemness potential.},
      keywords     = {Collagen I (Other) / Invasiveness (Other) / Matrigel
                      (Other) / Ring structures (Other) / Stemness (Other) / TROP2
                      (Other)},
      cin          = {MU01},
      ddc          = {610},
      cid          = {I:(DE-He78)MU01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40073643},
      doi          = {10.1016/j.prp.2025.155890},
      url          = {https://inrepo02.dkfz.de/record/299786},
}