%0 Journal Article
%A Gabele, Anna
%A Sprang, Maximilian
%A Cihan, Mert
%A Welzel, Mareen
%A Nurbekova, Assel
%A Romaniuk, Karolina
%A Dietzen, Sarah
%A Klein, Matthias
%A Bündgen, Georg
%A Emelianov, Maxim
%A Harms, Gregory
%A Rajalingam, Krishnaraj
%A Ziesmann, Tanja
%A Pape, Katrin
%A Wasser, Beatrice
%A Gomez-Zepeda, David
%A Braband, Kathrin
%A Delacher, Michael
%A Lemmermann, Niels
%A Bittner, Stefan
%A Andrade-Navarro, Miguel A
%A Tenzer, Stefan
%A Luck, Katja
%A Bopp, Tobias
%A Distler, Ute
%T Unveiling IRF4-steered regulation of context-dependent effector programs in CD4+ T cells under Th17- and Treg-skewing conditions.
%J Cell reports
%V 44
%N 3
%@ 2211-1247
%C Maryland Heights, MO
%I Cell Press
%M DKFZ-2025-00546
%P 115407
%D 2025
%Z HI-TRON
%X The transcription factor interferon regulatory factor 4 (IRF4) is crucial for the fate determination of pro-inflammatory T helper (Th) 17 and the functionally opposing group of immunomodulatory regulatory T (Treg) cells. However, the molecular mechanisms of how IRF4 steers diverse transcriptional programs in Th17 and Treg cells are far from being definitive. Here, we integrated data derived from affinity-purification and full mass-spectrometry-based proteome analysis with chromatin immunoprecipitation sequencing. This allowed the characterization of subtype-specific molecular programs and the identification of IRF4 interactors in the Th17/Treg context. Our data reveal that IRF4-interacting transcription factors are recruited to IRF composite elements for the regulation of cell-type-specific transcriptional programs as exemplarily demonstrated for FLI1, which, in cooperation with IRF4, promotes Th17-specific gene expression. FLI1 inhibition markedly impaired Th17 differentiation. The present 'omics' dataset provides a valuable resource for studying IRF4-mediated gene regulatory programs in pro- and anti-inflammatory immune responses.
%K CP: Immunology (Other)
%K ChIP-seq (Other)
%K FLI1 (Other)
%K IRF4 (Other)
%K Th17 cells (Other)
%K Treg cells (Other)
%K composite motif (Other)
%K protein-protein interaction (Other)
%K proteomics (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40067830
%R 10.1016/j.celrep.2025.115407
%U https://inrepo02.dkfz.de/record/299791