TY  - JOUR
AU  - Gabele, Anna
AU  - Sprang, Maximilian
AU  - Cihan, Mert
AU  - Welzel, Mareen
AU  - Nurbekova, Assel
AU  - Romaniuk, Karolina
AU  - Dietzen, Sarah
AU  - Klein, Matthias
AU  - Bündgen, Georg
AU  - Emelianov, Maxim
AU  - Harms, Gregory
AU  - Rajalingam, Krishnaraj
AU  - Ziesmann, Tanja
AU  - Pape, Katrin
AU  - Wasser, Beatrice
AU  - Gomez-Zepeda, David
AU  - Braband, Kathrin
AU  - Delacher, Michael
AU  - Lemmermann, Niels
AU  - Bittner, Stefan
AU  - Andrade-Navarro, Miguel A
AU  - Tenzer, Stefan
AU  - Luck, Katja
AU  - Bopp, Tobias
AU  - Distler, Ute
TI  - Unveiling IRF4-steered regulation of context-dependent effector programs in CD4+ T cells under Th17- and Treg-skewing conditions.
JO  - Cell reports
VL  - 44
IS  - 3
SN  - 2211-1247
CY  - Maryland Heights, MO
PB  - Cell Press
M1  - DKFZ-2025-00546
SP  - 115407
PY  - 2025
N1  - HI-TRON
AB  - The transcription factor interferon regulatory factor 4 (IRF4) is crucial for the fate determination of pro-inflammatory T helper (Th) 17 and the functionally opposing group of immunomodulatory regulatory T (Treg) cells. However, the molecular mechanisms of how IRF4 steers diverse transcriptional programs in Th17 and Treg cells are far from being definitive. Here, we integrated data derived from affinity-purification and full mass-spectrometry-based proteome analysis with chromatin immunoprecipitation sequencing. This allowed the characterization of subtype-specific molecular programs and the identification of IRF4 interactors in the Th17/Treg context. Our data reveal that IRF4-interacting transcription factors are recruited to IRF composite elements for the regulation of cell-type-specific transcriptional programs as exemplarily demonstrated for FLI1, which, in cooperation with IRF4, promotes Th17-specific gene expression. FLI1 inhibition markedly impaired Th17 differentiation. The present 'omics' dataset provides a valuable resource for studying IRF4-mediated gene regulatory programs in pro- and anti-inflammatory immune responses.
KW  - CP: Immunology (Other)
KW  - ChIP-seq (Other)
KW  - FLI1 (Other)
KW  - IRF4 (Other)
KW  - Th17 cells (Other)
KW  - Treg cells (Other)
KW  - composite motif (Other)
KW  - protein-protein interaction (Other)
KW  - proteomics (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40067830
DO  - DOI:10.1016/j.celrep.2025.115407
UR  - https://inrepo02.dkfz.de/record/299791
ER  -