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@ARTICLE{Gabele:299791,
author = {A. Gabele and M. Sprang and M. Cihan and M. Welzel and A.
Nurbekova and K. Romaniuk and S. Dietzen and M. Klein and G.
Bündgen and M. Emelianov and G. Harms and K. Rajalingam and
T. Ziesmann and K. Pape and B. Wasser and D.
Gomez-Zepeda$^*$ and K. Braband and M. Delacher and N.
Lemmermann and S. Bittner and M. A. Andrade-Navarro and S.
Tenzer$^*$ and K. Luck and T. Bopp and U. Distler},
title = {{U}nveiling {IRF}4-steered regulation of context-dependent
effector programs in {CD}4+ {T} cells under {T}h17- and
{T}reg-skewing conditions.},
journal = {Cell reports},
volume = {44},
number = {3},
issn = {2211-1247},
address = {Maryland Heights, MO},
publisher = {Cell Press},
reportid = {DKFZ-2025-00546},
pages = {115407},
year = {2025},
note = {HI-TRON},
abstract = {The transcription factor interferon regulatory factor 4
(IRF4) is crucial for the fate determination of
pro-inflammatory T helper (Th) 17 and the functionally
opposing group of immunomodulatory regulatory T (Treg)
cells. However, the molecular mechanisms of how IRF4 steers
diverse transcriptional programs in Th17 and Treg cells are
far from being definitive. Here, we integrated data derived
from affinity-purification and full mass-spectrometry-based
proteome analysis with chromatin immunoprecipitation
sequencing. This allowed the characterization of
subtype-specific molecular programs and the identification
of IRF4 interactors in the Th17/Treg context. Our data
reveal that IRF4-interacting transcription factors are
recruited to IRF composite elements for the regulation of
cell-type-specific transcriptional programs as exemplarily
demonstrated for FLI1, which, in cooperation with IRF4,
promotes Th17-specific gene expression. FLI1 inhibition
markedly impaired Th17 differentiation. The present 'omics'
dataset provides a valuable resource for studying
IRF4-mediated gene regulatory programs in pro- and
anti-inflammatory immune responses.},
keywords = {CP: Immunology (Other) / ChIP-seq (Other) / FLI1 (Other) /
IRF4 (Other) / Th17 cells (Other) / Treg cells (Other) /
composite motif (Other) / protein-protein interaction
(Other) / proteomics (Other)},
cin = {D190 / D191},
ddc = {610},
cid = {I:(DE-He78)D190-20160331 / I:(DE-He78)D191-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40067830},
doi = {10.1016/j.celrep.2025.115407},
url = {https://inrepo02.dkfz.de/record/299791},
}