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@ARTICLE{Li:299843,
      author       = {D. Li and Y. Yang and Z. Yin and L. Mao$^*$ and Y. Zhang
                      and P. Jiang and T. Zhu and T. He and X. Zhong and Q. Zheng
                      and W. Zhang},
      title        = {{CCCTC}-binding factor regulates splicing factor proline
                      and glutamine-rich to promote malignant growth of
                      osteosarcoma.},
      journal      = {American journal of translational research},
      volume       = {17},
      number       = {2},
      issn         = {1943-8141},
      address      = {Madison, Wis.},
      publisher    = {e-Century Publishing Corporation},
      reportid     = {DKFZ-2025-00580},
      pages        = {1495 - 1509},
      year         = {2025},
      note         = {Proteomics and Cancer Cell Signaling Group, German Cancer
                      Research Center (DKFZ), Heidelberg, 69120, Germany},
      abstract     = {CCCTC-binding factor (CTCF) is a candidate tumor regulatory
                      gene that encodes multifunctional transcription factors.
                      While its role in various cancers has been studied, its
                      function and mechanism in osteosarcoma were uncertain.
                      Previous studies have identified splicing factor proline and
                      glutamine-rich (SFPQ) as an oncogene in osteosarcoma.
                      Bioinformatic analysis suggested that CTCF may regulate SFPQ
                      transcriptionally. This study aimed to elucidate the role of
                      CTCF in osteosarcoma and explore its possible regulatory
                      relationship with SFPQ.Potential transcription factors of
                      SFPQ were identified using an online transcription factor
                      analysis database. The expression levels of CTCF in
                      osteosarcoma cells were assessed using quantitative
                      real-time PCR (qRT-PCR) and western blotting (WB). The
                      effect of CTCF and SFPQ on osteosarcoma cell behavior was
                      evaluated through cell function assays, dual-luciferase
                      reporter assays, and rescue experiments.Database analyses
                      (hTFtarget and GEPIA2) indicated a moderate correlation
                      between CTCF and SFPQ. qRT-PCR and WB results confirmed
                      significant CTCF expression in osteosarcoma cells.
                      Overexpression of CTCF enhanced cell proliferation,
                      migration, and invasion. Furthermore, CTCF was found to bind
                      to the promoter region of SFPQ, leading to its upregulation.
                      Rescue experiments demonstrated that SFPQ knockdown
                      attenuated the oncogenic effects of CTCF overexpression.CTCF
                      functions as an oncogene in osteosarcoma by positively
                      regulating SFPQ expression, thereby promoting the malignant
                      properties of osteosarcoma cells. These findings suggest
                      that targeting the CTCF-SFPQ axis may be a therapeutic
                      strategy for osteosarcoma.},
      keywords     = {CTCF (Other) / Osteosarcoma (Other) / SFPQ (Other) /
                      biological behavior (Other) / transcription factor (Other)},
      cin          = {A400},
      ddc          = {610},
      cid          = {I:(DE-He78)A400-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40092088},
      pmc          = {pmc:PMC11909532},
      doi          = {10.62347/STQK5435},
      url          = {https://inrepo02.dkfz.de/record/299843},
}