TY  - JOUR
AU  - Nguyen, Xuan Duc
AU  - Horn, Audrey
AU  - Fischer, Dania
AU  - Beck, Grietje
AU  - Spannenberger, Cora C
AU  - Gaudilliere, Brice
AU  - Horn, Jean-Louis
AU  - Thierse, Hermann-Josef
AU  - Frietsch, Thomas
TI  - Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study.
JO  - BMC anesthesiology
VL  - 25
IS  - 1
SN  - 1471-2253
CY  - [Erscheinungsort nicht ermittelbar]
PB  - BioMed Central
M1  - DKFZ-2025-00607
SP  - 129
PY  - 2025
N1  - Functional Proteome Analysis, German Cancer Research Center(DKFZ), Heidelberg 69120, Germany
AB  - It is questionable whether or not a short period of deep anesthesia can have long lasting effects on immune suppression.To analyze specific effects of deep anesthesia on immune modulation, a randomized-controlled, single-blinded study, monocentric, pilot-study was conducted at a level 1 orthopedic and trauma center. Inclusion criteria were patients scheduled for extended shoulder surgery with an ASA score between 1 to 3 (n = 186). Patients on immune modulating drugs or with immune deficits were excluded. The remaining patients were enrolled and randomized to either deep or light anesthesia (n = 18). Patient were randomized to receive either deep anesthesia or light anesthesia for 60 min or longer. The primary aim of the study was to compare cellular activity of T-cells, NK-cells and monocytes after anesthesia. Phagocytosis and cellular lysis activity of neutrophils and monocytes were analyzed by flow cytometry. Secondly, we analyzed anesthesia induced protein expresssion pattern in human monocytes by a standardized proteomic approach, implicating quantitative two-dimensional (2D) differential gel electrophoresis and Delta2D software analyses coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and Mascot analysis.Anesthesia duration was 109 min in the deep anesthesia group with 81 ± 17 min of BIS < 45 and a mean BIS of 38 ± 14. The light anesthesia group received anesthesia for 111 min with 13 ± 8 min of BIS < 45 and a mean BIS 56 ± 8. Cytotoxic T-cells decreased fivefold in the light anesthesia group compared to the deep anesthesia group (-28 ± 13
KW  - Humans
KW  - Pilot Projects
KW  - Male
KW  - Female
KW  - Middle Aged
KW  - Single-Blind Method
KW  - Immunity, Cellular: drug effects
KW  - Adult
KW  - Monocytes: metabolism
KW  - Monocytes: drug effects
KW  - Monocytes: immunology
KW  - Killer Cells, Natural: drug effects
KW  - Killer Cells, Natural: immunology
KW  - Phagocytosis: drug effects
KW  - Aged
KW  - Proteomics: methods
KW  - T-Lymphocytes: immunology
KW  - T-Lymphocytes: drug effects
KW  - Neutrophils: drug effects
KW  - Neutrophils: metabolism
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
KW  - Anesthesia depth (Other)
KW  - Cellular immune response (Other)
KW  - Lymphocyte proliferation (Other)
KW  - Monocyte proteome (Other)
KW  - NK-cells (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40097954
C2  - pmc:PMC11912595
DO  - DOI:10.1186/s12871-025-02980-9
UR  - https://inrepo02.dkfz.de/record/300109
ER  -