%0 Journal Article
%A Patel, Areeba Jamilkhan
%A Göbel, Kirsten
%A Ille, Sebastian
%A Hinz, Felix
%A Schoebe, Natalie
%A Bogumil, Henri
%A Meyer, Jochen
%A Brehm, Michelle
%A Kardo, Helin
%A Schrimpf, Daniel
%A Lomakin, Artem
%A Ritter, Michael
%A Göller, Pauline
%A Kerbs, Paul
%A Pfeifer, Lisa
%A Hamelmann, Stefan
%A Blume, Christina
%A Ippen, Franziska
%A Berghaus, Natalie
%A Euskirchen, Philipp
%A Schweizer, Leonille
%A Hultschig, Claus
%A Van Roy, Nadine
%A Van Dorpe, Jo
%A Van der Meulen, Joni
%A Loontiens, Siebe
%A Dedeurwaerdere, Franceska
%A Leske, Henning
%A Halldórsson, Skarphéðinn
%A Fox, Graeme
%A Deacon, Simon
%A Cahyani, Inswasti
%A Holmes, Nadine
%A Wibowo, Satrio
%A Munro, Rory
%A Martin, Dan
%A Sharif, Abid
%A Housley, Mark
%A Goldspring, Robert
%A Brandner, Sebastian
%A Roy, Somak
%A Hench, Jürgen
%A Frank, Stephan
%A Unterberg, Andreas
%A Goidts, Violaine
%A Jäger, Natalie
%A Paine, Simon
%A Smith, Stuart
%A Herold-Mende, Christel
%A Wick, Wolfgang
%A Pfister, Stefan
%A Vik-Mo, Einar O
%A von Deimling, Andreas
%A Krieg, Sandro
%A Jones, David Tw
%A Loose, Matthew
%A Schlesner, Matthias
%A Sill, Martin
%A Sahm, Felix
%T Prospective, multicenter validation of a platform for rapid molecular profiling of central nervous system tumors.
%J Nature medicine
%V 31
%N 5
%@ 1078-8956
%C [New York, NY]
%I Springer Nature
%M DKFZ-2025-00638
%P 1567-1577
%D 2025
%Z #EA:B300#LA:B300#LA:B062# / 2025 May;31(5):1567-1577
%X Molecular data integration plays a central role in central nervous system (CNS) tumor diagnostics but currently used assays pose limitations due to technical complexity, equipment and reagent costs, as well as lengthy turnaround times. We previously reported the development of Rapid-CNS2, an adaptive-sampling-based nanopore sequencing workflow. Here we comprehensively validated and further developed Rapid-CNS2 for intraoperative use. It now offers real-time methylation classification and DNA copy number information within a 30-min intraoperative window, followed by comprehensive molecular profiling within 24 h, covering the complete spectrum of diagnostically and therapeutically relevant information for the respective entity. We validated Rapid-CNS2 in a multicenter setting on 301 archival and prospective samples including 18 samples sequenced intraoperatively. To broaden the utility of methylation-based CNS tumor classification, we developed MNP-Flex, a platform-agnostic methylation classifier encompassing 184 classes. MNP-Flex achieved 99.6
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40133526
%R 10.1038/s41591-025-03562-5
%U https://inrepo02.dkfz.de/record/300147